About: Flurescence microscopy in biological and medical research

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Projekt, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Projekt
rdfs:seeAlso	http://www.isvav.cz/projectDetail.do?rowId=LC06063
Description	The main objective is development of new methods in fluorescence microscopy for investigation of structure and dynamics of living cells and model systems. The methodological part contains experimental techniques (FLIM, FCS, super-resolution microscopy, electrochemistry and their combinations) and numerical methods for image processing. The application part focuses on development of carriers for non-viral gene therapy, elucidation of the role of G proteins in hormonal action regulation, investigation of proteins involved in cell nucleus organization, explanation of protein and peptide interaction with model membranes and understanding the role of membrane transporters in yeast colonies formation. Simultaneously, a closer connection between biological and physical laboratories is being formed leading to a more efficient use of modern microscopic techniques in solving current biological problems. (en) Hlavním cílem je vývoj nových metod ve fluorescenční mikroskopii pro studium struktury a dynamiky živých buněk a modelových systémů. Metodologická stránka zahrnuje experimentální techniky (FLIM, FCS, mikroskopie se subdifrakčním rozlišením, elektrochemické metody a jejich kombinace) a numerické metody zpracování obrazových dat. Aplikační stránka se soustředí na vývoj nosičů pro nevirální genetickou terapii, objasnění role G proteinů v regulaci hormonální akce, studium interakcí proteinů podílejících se na organizaci buněčného jádra, vysvětlení interakce proteinů a peptidů s modely biologických membrán či porozumění role membránových transportérů ve tvorbě kvasinkových kolonií. Současně dochází k užšímu propojení biologických a fyzikálních pracovišť vedoucímu k efektivnímu využití nejmodernějších mikroskopických technik při řešení aktuálních biologický problémů.
Title	Flurescence microscopy in biological and medical research (en) Fluorescenční mikroskopie v biologickém a lékařském výzkumu
skos:notation	LC06063
http://linked.open...avai/cep/aktivita	Basic Research Centres
http://linked.open...kovaStatniPodpora	http://linked.opendata.cz/resource/domain/vavai/projekt/LC06063/celkovaStatniPodpora
http://linked.open...ep/celkoveNaklady	http://linked.opendata.cz/resource/domain/vavai/projekt/LC06063/celkoveNaklady
http://linked.open...datumDodatniDoRIV	2012-07-03 (xsd:date)
http://linked.open...i/cep/druhSouteze	VS - Veřejná soutěž ve výzkumu a vývoji
http://linked.open...ep/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open.../cep/fazeProjektu	91883515
http://linked.open...ai/cep/hlavniObor	EB - Genetika a molekulární biologie
http://linked.open...hodnoceniProjektu	V - Vynikající výsledky (s mezinárodním významem apod.). Zároveň byly splněny cíle a předpokládané výsledky uvedené ve smlouvě / rozhodnutí o poskytnutí podpory.
http://linked.open...vai/cep/kategorie	ZV - Základní výzkum
http://linked.open.../cep/klicovaSlova	fluoroscenční mikroskopie, jednotlivá molekula, živé buňky, FRET, FCS, FRAP, časově rozlišená fluorescence, DNA, fosfolipidy, ITIES, receptory spřažené s G-proteiny, neuropřenašeč, kvasinkové transportní proteiny, rafty plasmatické membrány, analýza obra (en)
http://linked.open...ep/partnetrHlavni	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...inujicichPrijemcu	1 (xsd:int)
http://linked.open...cep/pocetPrijemcu	4 (xsd:int)
http://linked.open...ocetSpoluPrijemcu	0 (xsd:int)
http://linked.open.../pocetVysledkuRIV	170 (xsd:int)
http://linked.open...enychVysledkuVRIV	170 (xsd:int)
http://linked.open...lneniVMinulemRoce	2011-02-11 (xsd:date)
http://linked.open.../prideleniPodpory	http://linked.opendata.cz/resource/domain/vavai/cep/prideleniPodpory/32341%2F2010-31
http://linked.open...iciPoslednihoRoku	2011
http://linked.open...atUdajeProjZameru	2012
http://linked.open.../vavai/cep/soutez	SMSM200600LC2
http://linked.open...usZobrazovaneFaze	DUU
http://linked.open...ai/cep/typPojektu	P - Projekt výzkumu a vývoje financovaný ze státního rozpočtu
http://linked.open...ep/ukonceniReseni	2011-12-31 (xsd:date)
http://linked.open.../cep/vedlejsiObor	CF - Fyzikální chemie a teoretická chemie BO - Biofyzika
http://linked.open...ep/zahajeniReseni	2006-03-01 (xsd:date)
http://linked.open...jektu+dodavatelem	Nové přístrojové vybavení instalované v laboratořích centra umožňuje používání nejpokročilejších technik fluorescenční mikroskopie, z nichž některé jsou unikátní ve světovém měřítku. Současně byly vyvíjeny nové algoritmy pro zpracování obrazu. Tyto přístupy byly aplikovány na aktuální biologické problémy a výstupy byly publikovány v prestižních časopisech. (cs) New instrumentation, installed in laboratories of the centre, allows implementation of the most advanced techniques of fluorescence microscopy, some being unique on the international level. In parallel, novel algorithms of image processing have been developed. Biological issues of current importance have been addressed by the new approaches and obtained results published in prestigious journals. (en)
http://linked.open...tniCyklusProjektu	ZBBBBKU
http://linked.open.../cep/klicoveSlovo	DNA FCS fosfolipidy FRAP FRET ITIES jednotlivá molekula kvasinkové transportní proteiny neuropřenašeč rafty plasmatické membrány receptory spřažené s G-proteiny časově rozlišená fluorescence živé buňky fluoroscenční mikroskopie
is http://linked.open...vavai/riv/projekt of	Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription Porphyrin/calixarene self-assemblies in aqueous solution New organic FET-like photoactive device, experiments and DFT modeling DFT modeling and experiments on phthalocyanines field-effect transistors Spectral analysis of doxorubicin accumulation and the indirect quantification of its DNA intercalation Surface expression of metabotropic glutamate receptor variants mGluR1a and mGluR1b in transfected HEK293 cells In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer Probing filamentarity of nuclear skeletal structures by methods of spatial statistics, mathematical morphology and image analysis The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells Two photon excitation microscopy and SHG imaging as a tool for visualization of type I and type II collagen, and their use in tissue engineering Proton transfer across a liquid/liquid interface facilitated by phospholipid interfacial flims Testate Amoebae (Arcellinida & Euglyphida) vs. ericoid mycorrhizal and DSE fungi: a possible novel interaction in the mycorrhizosphere of ericaceous plants? Role of distinct dimorphic transitions in territory colonizing and formation of yeast colony architecture. Applications of phasors to in vitro time-resolved fluorescence measurements Applications of phasor plots to in vitro protein studies Formation of arenicin-1 microdomains in bilayers and their specific lipid interaction revealed by Z-scan FCS Fluorescence spectroscopy studies of HEK293 cells expressing DOR-Gi1alfa fusion protein; the effect of cholesterol depletion Flo11p, drug efflux pumps, and the extracellular matrix cooperate to form biofilm yeast colonies Mass Spectrometric Characterization of Oligomers in Pseudomonas aeruginosa Azurin Solutions Mapping of geometrical characteristics and 3D visualization of blood capillary network from confocal microscopic images Monolayers at electrodes as basis for models of natural membranes Oxidized Phosphatidylcholines Facilitate Phospholipid Flip-Flop in Liposomes Preparation and Properties of Reference Electrodes Based on Silver Paste Amalgam STIM1-Directed Reorganization of Microtubules in Activated Mast Cells Unusual reaction of gold on Hanging Mercury Drop Electrode Up-regulation of adenylylcyclases I and II induced by long-term adaptation of rats to morphine fades away 20 days after morphine withdrawal Probing diffusion laws within cellular membranes by Z-scan fluorescence correlation spectroscopy Fluorescence lifetime correlation spectroscopy combined with lifetime tuning: New perspectives in supported phospholipid bilayer research Headgroup hydration and mobility of DOTAP/DOPC bilayers: A fluorescence solvent relaxation study Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions Voltammetric Determination of Nitrophenols at a Silver Solid Amalgam Electrode Fluorescence lifetime correlation spectroscopy Confined diffusion in ordered nanoporous alumina membranes The use of solvent relaxation technique to investigate headgroup hydration and protein binding of simple and mixed phosphatidylcholine/surfactant bilayer membranes Molecular interpretation of fluorescence solvent relaxation of Patman and 2H NMR experiments in phosphatidylcholine bilayers Self-organization of water in lithium/nitrobenzene system Adsorption and ion-pairing interactions of phospholipids in the system of two immiscible electrolyte solutions. Part II: ... Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes Fluorescence correlation spectroscopic studies of a single lipopolyamine-DNA nanoparticle Disruption of the Plasma Membrane Integrity by Cholesterol Depletion Impairs Effectiveness of TRH Receptor-Mediated Signal Transduction via Gq/G11α Proteins Adsorption and ion-pairing interactions of phospholipids in the system of two immiscible electrolyte solutions - Part I. The behaviour of lecithin at the water/1,2-dichloroethane interface, compared with that of trimethyloctadecylammonium cation Adsorption and ion-pairing interactions of phospholipids in the system of two immiscible electrolyte solutions. Part II: The formation and behaviour of a lecithin layer at the water/1,2-dichloroethane interface in the presence of multivalent anions in... Diversity in muscle fibre type cappilary supply Novel efficient methods for measuring mesophyll anatomical characteristics from fresh thick sections using stereology and confocal microscopy: application on acid rain-treated Norway spruce needles LP-12 Approaches to visualization of 3D structures captured by a confocal microscope Adaptation of muscle fibre types and capillary network to acute denervation and shortlasting reinnervation Surface density and volume density measurements of chloroplast thylakoids in maize ( Zea mays L.) under chilling conditions Disruption of the Plasma Membrane Integrity by Cholesterol Depletion Impairs Effectiveness of TRH Receptor-Mediated Signal Transduction via Gq/G11 Proteins

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