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Description
  • Fluorescence antibunching is a well-known technique for determining the number of independent emitters per molecule or molecular complex. It was rarely applied to autofluorescent proteins due to the necessity of collecting large numbers of fluorescence photons from a single molecule, which is usually impossible to achieve with rather photolabile autofluorescent proteins. Here, we measure fluorescence antibunching on molecules in solution, allowing us to accumulate data over a large number of molecules. We use that method for determining an average stoichiometry of molecular complexes. The proposed method is absolute in the sense that it does not need any calibration or referencing. We develop the necessary theoretical background and check the method on pure dye solutions and on molecular complexes with known stoichiometry.
  • Fluorescence antibunching is a well-known technique for determining the number of independent emitters per molecule or molecular complex. It was rarely applied to autofluorescent proteins due to the necessity of collecting large numbers of fluorescence photons from a single molecule, which is usually impossible to achieve with rather photolabile autofluorescent proteins. Here, we measure fluorescence antibunching on molecules in solution, allowing us to accumulate data over a large number of molecules. We use that method for determining an average stoichiometry of molecular complexes. The proposed method is absolute in the sense that it does not need any calibration or referencing. We develop the necessary theoretical background and check the method on pure dye solutions and on molecular complexes with known stoichiometry. (en)
  • Fluorescenční antibunching je známá metoda pro určování počtu nezávislých emiterů přítomných v molekulárních komplexech. Tato metoda byla ale zřídka aplikována pro studium autofluorescenčních proteinů imobilizovaných na povrchu kvůli jejich nízké fotostabilitě. V této práci jsme měřili fluorescenční antibunching v roztoku a nasbírali tak data z obrovského počtu molekul, což nám umožnilo určit stechiometrii komplexů těchto fotolabilních proteinů. Tato metoda navíc nevyžaduje kalibraci standardem. (cs)
Title
  • Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes
  • Měření “antibunchingu“ fluorescence v roztoku: cesta k určení stechiometrie molekulovýxch komplexů (cs)
  • Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes (en)
skos:prefLabel
  • Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes
  • Měření “antibunchingu“ fluorescence v roztoku: cesta k určení stechiometrie molekulovýxch komplexů (cs)
  • Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes (en)
skos:notation
  • RIV/61388955:_____/07:00089208!RIV08-MSM-61388955
http://linked.open.../vavai/riv/strany
  • 4040;4049
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LC06063), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika
  • -
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 421348
http://linked.open...ai/riv/idVysledku
  • RIV/61388955:_____/07:00089208
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • exploring fluorescence antibunching; molecular complexes; biophysical methods (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [4E3C98155462]
http://linked.open...i/riv/nazevZdroje
  • Analytical Chemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 79
http://linked.open...iv/tvurceVysledku
  • Sýkora, Jan
  • Kaiser, K.
  • Gregor, I.
  • Enderlain, J.
  • Bönigk, W.
  • Schmalzing, G.
http://linked.open...n/vavai/riv/zamer
issn
  • 0003-2700
number of pages
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