About: In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer

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About: In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Expression of the nascent RNA transcript is regulated by its interaction with a number of proteins. Misregulation of such interactions often results in impaired cellular functions that can lead to cancer and many diseases. Our understanding of RNA–protein interaction within the cellular context is thus essential for development of novel diagnostic and therapeutic tools. While there are many in vitro methods analyzing RNA–protein interactions, in vivo approaches are scarce. Here we established a method based on fluorescence resonance energy transfer (FRET), which we termed RNA-binding mediated FRET (RB-FRET). The methods enables determination of RNA–protein interaction inside cells and was tested on hnRNP H protein binding to its cognate RNA. Using two different approaches, we provided evidence that RB-FRET was sensitive enough to detect specific RNA–protein interactions in cell, providing a powerful tool to study spatial and temporal localization of specific RNA–protein complexes. Expression of the nascent RNA transcript is regulated by its interaction with a number of proteins. Misregulation of such interactions often results in impaired cellular functions that can lead to cancer and many diseases. Our understanding of RNA–protein interaction within the cellular context is thus essential for development of novel diagnostic and therapeutic tools. While there are many in vitro methods analyzing RNA–protein interactions, in vivo approaches are scarce. Here we established a method based on fluorescence resonance energy transfer (FRET), which we termed RNA-binding mediated FRET (RB-FRET). The methods enables determination of RNA–protein interaction inside cells and was tested on hnRNP H protein binding to its cognate RNA. Using two different approaches, we provided evidence that RB-FRET was sensitive enough to detect specific RNA–protein interactions in cell, providing a powerful tool to study spatial and temporal localization of specific RNA–protein complexes. (en)
Title	In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer (en)
skos:prefLabel	In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer (en)
skos:notation	RIV/68378050:_____/09:00334099!RIV10-MSM-68378050
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(KAN200520801), P(LC06063), Z(AV0Z40400503), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Huranová, Martina Staněk, David
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	318932
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/09:00334099
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	FRET; FLIM; RNA-protein interactions (en)
http://linked.open.../riv/klicoveSlovo	FRET FLIM RNA-protein interactions
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[DC128D3C8B2E]
http://linked.open...i/riv/nazevZdroje	R N A
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Flurescence microscopy in biological and medical research Targeted expression and transport of bioactive molecules
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	15
http://linked.open...iv/tvurceVysledku	Hof, Martin Staněk, David Benda, Aleš Huranová, Martina Caputi, M. Jablonski, J. A
http://linked.open...ain/vavai/riv/wos	000270851000014
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	1355-8382
number of pages	9 (xsd:int)
is http://linked.open...avai/riv/vysledek of	In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer

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