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rdf:type
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Description
| - Difúze fluorescenčně značeného lipidu byla změřena pro dva modelové systémy, a to pro obří unilamelární vesikly (GUVs) a pro podporované fosfolipidové membrány (SPBs). Pro měření difúzního koeficientu byla použita „Z-scan“ fluorescenční korelační spektroskopie (FCS). Zvláštní pozornost byla věnována tomu, aby se nelišily podmínky měření pro oba modelové systémy a obdržené hodnoty tak mohly být kvantitativně porovnány. Srovnání difúzního koeficientu pro GUVs (D = 7.8 +/- 0.8 mu m(2) s(-1)) a SPBs (D = 3.1+/- 0.3 mu m(2) s(-1)) jasně ukazuje, že difúze lipidů v GUVs je zhruba dvakrát rychlejší než v SPBs. (cs)
- The lateral diffusion coefficients of a BODIPY tail-labeled lipid in two model systems, namely, free-standing giant unilamellar vesicles (GUVs) and supported phospholipid bilayers (SPBs), were determined by fluorescence correlation spectroscopy (FCS) using the Z-scan approach. For the first time, the performed measurements on 1,2-dioleoyl-sn-glycero-3-phosphocholine ( DOPC) bilayers maintain exactly the same experimental conditions for both systems, which allows for a quantitative comparison of lipid diffusion in these two commonly used model membranes. The results obtained revealed that the lipid mobility in free-standing bilayers (D = 7.8 +/- 0.8 mu m(2) s(-1)) is significantly higher than in the bilayer created on the solid support (mica) (D = 3.1 +/- 0.3 mu m(2) s(-1)).
- The lateral diffusion coefficients of a BODIPY tail-labeled lipid in two model systems, namely, free-standing giant unilamellar vesicles (GUVs) and supported phospholipid bilayers (SPBs), were determined by fluorescence correlation spectroscopy (FCS) using the Z-scan approach. For the first time, the performed measurements on 1,2-dioleoyl-sn-glycero-3-phosphocholine ( DOPC) bilayers maintain exactly the same experimental conditions for both systems, which allows for a quantitative comparison of lipid diffusion in these two commonly used model membranes. The results obtained revealed that the lipid mobility in free-standing bilayers (D = 7.8 +/- 0.8 mu m(2) s(-1)) is significantly higher than in the bilayer created on the solid support (mica) (D = 3.1 +/- 0.3 mu m(2) s(-1)). (en)
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Title
| - Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions
- Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions (en)
- Difúze lipidů v obřích unilamelárních vesiklech je více než dvakrát rychlejší než difúze v podporovaných fosfolipidových membránách za totožných podmínek (cs)
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skos:prefLabel
| - Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions
- Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions (en)
- Difúze lipidů v obřích unilamelárních vesiklech je více než dvakrát rychlejší než difúze v podporovaných fosfolipidových membránách za totožných podmínek (cs)
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skos:notation
| - RIV/61388955:_____/06:00043210!RIV07-AV0-61388955
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA203/05/2308), P(LC06063), Z(AV0Z40400503)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61388955:_____/06:00043210
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - fluorescence-correlation spectroscopy; model membranes; molecular friction (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Hof, Martin
- Sýkora, Jan
- Humpolíčková, Jana
- Benda, Aleš
- Przybylo, Magdalena
- Zan, Anna
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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is http://linked.open...avai/riv/vysledek
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