About: The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Precursor messenger RNA (pre-mRNA) splicing is catalyzed by the spliceosome, a large ribonucleoprotein (RNP) complex composed of five small nuclear RNP particles (snRNPs) and additional proteins. Using live cell imaging of GFP-tagged snRNP components expressed at endogenous levels, we examined how the spliceosome assembles in vivo. A comprehensive analysis of snRNP dynamics in the cell nucleus enabled us to determine snRNP diffusion throughout the nucleoplasm as well as the interaction rates of individual snRNPs with pre-mRNA. Core components of the spliceosome, U2 and U5 snRNPs, associated with pre-mRNA for 15-30 s, indicating that splicing is accomplished within this time period. Additionally, binding of U1 and U4/U6 snRNPs with pre-mRNA occurred within seconds, indicating that the interaction of individual snRNPs with pre-mRNA is distinct. Precursor messenger RNA (pre-mRNA) splicing is catalyzed by the spliceosome, a large ribonucleoprotein (RNP) complex composed of five small nuclear RNP particles (snRNPs) and additional proteins. Using live cell imaging of GFP-tagged snRNP components expressed at endogenous levels, we examined how the spliceosome assembles in vivo. A comprehensive analysis of snRNP dynamics in the cell nucleus enabled us to determine snRNP diffusion throughout the nucleoplasm as well as the interaction rates of individual snRNPs with pre-mRNA. Core components of the spliceosome, U2 and U5 snRNPs, associated with pre-mRNA for 15-30 s, indicating that splicing is accomplished within this time period. Additionally, binding of U1 and U4/U6 snRNPs with pre-mRNA occurred within seconds, indicating that the interaction of individual snRNPs with pre-mRNA is distinct. (en)
Title	The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells (en)
skos:prefLabel	The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells (en)
skos:notation	RIV/68378050:_____/10:00351126!RIV11-GA0-68378050
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/07/0133), P(GAP305/10/0424), P(KAN200520801), P(LC06063), Z(AV0Z40400503), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Huranová, Martina Staněk, David Ivani, Ivan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	254383
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/10:00351126
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	FRAP; RNA splicing; spliceosome (en)
http://linked.open.../riv/klicoveSlovo	FRAP spliceosome RNA splicing
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[2F7DBF7FAC7D]
http://linked.open...i/riv/nazevZdroje	Journal of Cell Biology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Self-organization principles of non-membrane-bound organels in eukaryotic cells Flurescence microscopy in biological and medical research Targeted expression and transport of bioactive molecules Regulation of alternative splicing via chromatin acetylation
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	191
http://linked.open...iv/tvurceVysledku	Hof, Martin Staněk, David Benda, Aleš Huranová, Martina Poser, I. Ivani, Ivan Brody, Y. Neugebauer, K. M. Shav-Tal, Y.
http://linked.open...ain/vavai/riv/wos	000282648500009
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0021-9525
number of pages	12 (xsd:int)
is http://linked.open...avai/riv/vysledek of	The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells

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