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| Description
| - The project goal is a comparative study of bioenergetics pathways, signaling mechanisms and membrane functions of thawed sperm during motility observation using chondrostean and teleostean fishes. We will demonstrate that according to process of cryopreservation and thawing of sperm, membrane permeability and dyneins/microtubules reactivity flagellum would become changed. On the base of these studies we will try to find possible species specific proteins responsible for flagellar regulatory mechanisms at chondrostean and teleostean fishes and find correlation between thawed motile sperm and protein structure of the flagellum. Firstly we will try to understand and to explain why duration of motility is affected after cryopreservation by evaluation of ATP, ADP and PCr level and also cryopreserved demembranated spermatozoa with studdying cryobiology machinery of microtubules with midepiece in both model species. (en)
- Cílem projektu je komparativní studie bioenergetických drah, signálních mechanismů a funkce membrány v průběhu motility rozmrazených spermií u chrupavčitých a kostnatých ryb. Budeme se snažit ukázat, že na základě zmrazení a rozmrazení spermií dojde ke změnám permeability membrány, včetně dyneinové-mikrotubulové reaktivity bičíku. Pokusíme se najít druhově specifický protein regulující pohyb bičíku spermií u chrupavčitých a kostnatých ryb a rovněž nalézt korelaci mezi motilitou rozmrazených spermií a proteinovou strukturou bičíku u těchto druhů. Poprvé se budeme snažit u obou modelových druhů vysvětlit proč doba motility spermií je ovlivněna zmrazováním a to vyhodnocením úrovně ATP, ADP a PCr a rovněž se pokusíme zmrazit demembranované spermie pro studium kryobility mechanismu funkčnosti mikrotubulů, včetně střední části spermie.
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| Title
| - Diversity of Bioenergetics Pathways, Membrane Functions, Signaling Mechanisms and Proteomics of Cryopreserved Sperm of Evolutionary Different Fish Species (en)
- Diverzita bioenergetických drah, funkce membrány, signálních mechanismů a proteomiky zmrazeného spermatu evolučně rozdílných druhů ryb
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| skos:notation
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| http://linked.open...avai/cep/aktivita
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| http://linked.open...kovaStatniPodpora
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| http://linked.open...ep/celkoveNaklady
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| http://linked.open...datumDodatniDoRIV
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| http://linked.open...i/cep/druhSouteze
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| http://linked.open...ep/duvernostUdaju
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| http://linked.open.../cep/fazeProjektu
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| http://linked.open...ai/cep/hlavniObor
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| http://linked.open...hodnoceniProjektu
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| http://linked.open...vai/cep/kategorie
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| http://linked.open.../cep/klicovaSlova
| - cryopreservation; spermatozoa; bičík; ryby; kapr; jeseter; energie; ATP, proteins (en)
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| http://linked.open...ep/partnetrHlavni
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| http://linked.open...inujicichPrijemcu
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| http://linked.open...cep/pocetPrijemcu
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| http://linked.open...ocetSpoluPrijemcu
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| http://linked.open.../pocetVysledkuRIV
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| http://linked.open...enychVysledkuVRIV
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| http://linked.open...lneniVMinulemRoce
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| http://linked.open.../prideleniPodpory
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| http://linked.open...iciPoslednihoRoku
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| http://linked.open...atUdajeProjZameru
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| http://linked.open.../vavai/cep/soutez
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| http://linked.open...usZobrazovaneFaze
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| http://linked.open...ai/cep/typPojektu
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| http://linked.open...ep/ukonceniReseni
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| http://linked.open.../cep/vedlejsiObor
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| http://linked.open...ep/zahajeniReseni
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| http://linked.open...jektu+dodavatelem
| - Sperm physico-chemical properties, protein profiles, cryoresistance and fertilizing ability were studied in sturgeons and bony fish species. For five years was published 31 articles in journals with IF from 0.7 to 3.3 and a chapter in the book. (en)
- Fyzikálně-chemické vlastnosti spermií, jejich proteinové profily, cryoresistance a oplozovací schopnost byla studována u jeseterů a kostnatých rybích druhů. Za pět let bylo publikováno 31 článků v časopisech s IF od 0,7 do 3,3 a jedna kapitola v knize. (cs)
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| http://linked.open...tniCyklusProjektu
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| http://linked.open.../cep/klicoveSlovo
| - cryopreservation
- ATP
- bičík
- energie
- jeseter
- kapr
- ryby
- spermatozoa
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| is http://linked.open...vavai/riv/projekt
of | - Effect of short-term storage on quality parameters, DNA integrity, and oxidative stress in Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeon sperm
- Motility of sturgeon spermatozoa can sustain successive activations episodes
- Pre-spawning water temperature affects sperm respiration and reactivation parameters in male carps
- Freeze-thawing as the factor of spontaneous activation of spermatozoa motility in common carp (Cyprinus carpio L.)
- Effects of osmolality on sperm morphology, motility and flagellar wave parameters in Northen pike (Esox lucius L.)
- Dynamics of ATP and movement in Eurasian perch (Perca fluviatilis L.) sperm in conditions of decreasing osmolality
- Sperm proteins in teleostean and chondrostean (sturgeon) fishes
- Ultrastructural study on the fertilisation process in sturgeon (Acipenser), function of acrosome and prevention of polyspermy
- Sperm motility and monthly variations of semen characteristics in Perca fluviatilis (Teleostei: Percidae)
- Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L
- Dietary n-3/n-6 ratio affects the biochemical composition of Eurasian perch (Perca fluviatilis) semen but not indicators of sperm quality
- Cryopreservation of Atlantic cod Gadus morhua L. spermatozoa: Effects of extender composition and freezing rate on sperm motility, velocity and morphology
- Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso
- Spontaneous activation of spermatozoa motility by routine freeze-thawing in different fish species
- In vitro effects of Bisphenol A on sperm motility characteristics in Perca fluviatilis L. (Percidae; Teleostei)
- Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques
- Percoll gradient separation of cryopreserved common carp spermatozoa to obtain a fraction with higher motility, velocity and membrane integrity
- Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces)
- Potential role of the acrosome of sturgeon spermatozoa in the fertilization process
- Roles of osmolality, calcium - Potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm
- Sperm morphology, ATP content, and analysis of motility in Atlantic halibut (Hippoglossus hippoglossus)
- Spermatozoa Concentration, Seminal Plasma Composition and Their Physiological Relationship in the Endangered Stellate Sturgeon (Acipenser stellatus) and Russian Sturgeon (Acipenser gueldenstaedtii)
- Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants
- Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus)
- Different swimming behaviors of sterlet (Acipenser ruthenus) spermatozoa close to solid and free surfaces
- Fins improve the swimming performance of fish sperm: A hydrodynamic analysis of the Siberian sturgeon Acipenser baerii
- The in vitro effect of duroquinone on functional competence, genomic integrity, and oxidative stress indices of sterlet (Acipenser ruthenus) spermatozoa
- Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa
- Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa
- In vitro effects of bisphenol A on the quality parameters, oxidative stress, DNA integrity and adenosine triphosphate content in sterlet (Acipenser ruthenus) spermatozoa
- Staining of sturgeon spermatozoa with trypsin inhibitor from soybean, Alexa Fluor? 488 conjugate for visualization of sturgeon acrosome
- Evaluation of Spermiation Indices with Multiple Sperm Collections in Endangered Sterlet (Acipenser ruthenus)
- Morphology and fine structure of the Russian sturgeon, Acipenser gueldenstaedtii (Acipenseridae, Chondrostei) spermatozoa
- Sperm biology and control of reproduction in sturgeon: (I) testicular development, sperm maturation and seminal plasma characteristics
- Spermatozoa motility, cryoresistance, and fertilizing ability in sterlet Acipenser ruthenus during sequential stripping
- Sperm morphology and biology in sturgeon
- Spermatozoa Motility and Variation in the Seminal Plasma Proteome of Eurasian Perch (Perca fluviatilis) During the Reproductive Season
- Sperm biology and control of reproduction in sturgeon: (II) sperm morphology, acrosome reaction, motility and cryopreservation
- Sperm characteristics and androgens in Acipenser ruthenus after induction of spermiation by carp pituitary extract or GnRHa implants
- Biological characteristics of sperm in two oyster species: the Pacific Oyster, Crassostrea gigas, and the Black-Lip Pearl Oyster, Pinctada margaritifera
- Mechanism of action of mercury on sperm morphology, Adenosine triphosphate content, and motility in Perch (Perca fluviatilis) (Percidae; Teleostei)
- Physico-chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)
- Opakovaný odběr spermatu jesetera malého a jeho využití při řízené reprodukci
- Regulation of spermatozoa motility in response to cations in Russian sturgeon Acipenser gueldenstaedtii
- Wave propagation in flagella
- Atp, the Sperm Movement Energizer
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| is http://linked.open...vavai/cep/projekt
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