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Description
| - The present study shows the roles of osmolality, Ca2+- K+ antagonist and Ca2+ in sperm activation and flagellar beating of sturgeon. Sperm motility was activated at hypoosmolality and suppressed at 175 mOsmol kg?1. Sperm activation was suppressed by 0.35mM K+, but Ca2+ could reverse K+ inhibitory effect at Ca2+: K+ ratio of 0.25. Neither EGTA (a chelator of Ca2+) nor nifedipine (a Ca2+ channel blocker) prevented sperm activation. But, sperm motility and velocity were decreased by EGTA, nifedipine and an inhibitor for Ca2+/calmodulin protein (w-7); suggesting role of Ca2+ signaling after triggering sperm activation through hypoosmolality. Symmetric flagellar beating was turned to asymmetric after activation in w-7; evidence for modulation of Ca2+-binding proteins activity. Sturgeon sperm, similar to salmonids, is immotile in seminal plasma due to high K+ , but the mechanism of sperm activation seems to be closer to freshwater teleosts, where osmolality prohibits sperm activation in seminal plasma.
- The present study shows the roles of osmolality, Ca2+- K+ antagonist and Ca2+ in sperm activation and flagellar beating of sturgeon. Sperm motility was activated at hypoosmolality and suppressed at 175 mOsmol kg?1. Sperm activation was suppressed by 0.35mM K+, but Ca2+ could reverse K+ inhibitory effect at Ca2+: K+ ratio of 0.25. Neither EGTA (a chelator of Ca2+) nor nifedipine (a Ca2+ channel blocker) prevented sperm activation. But, sperm motility and velocity were decreased by EGTA, nifedipine and an inhibitor for Ca2+/calmodulin protein (w-7); suggesting role of Ca2+ signaling after triggering sperm activation through hypoosmolality. Symmetric flagellar beating was turned to asymmetric after activation in w-7; evidence for modulation of Ca2+-binding proteins activity. Sturgeon sperm, similar to salmonids, is immotile in seminal plasma due to high K+ , but the mechanism of sperm activation seems to be closer to freshwater teleosts, where osmolality prohibits sperm activation in seminal plasma. (en)
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Title
| - Roles of osmolality, calcium - Potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm
- Roles of osmolality, calcium - Potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm (en)
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skos:prefLabel
| - Roles of osmolality, calcium - Potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm
- Roles of osmolality, calcium - Potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm (en)
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skos:notation
| - RIV/60076658:12520/11:43876864!RIV12-GA0-12520___
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http://linked.open...avai/predkladatel
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(ED2.1.00/01.0024), P(GA523/09/1793), P(GAP502/11/0090), P(IAA608030801), P(ME10015), S
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12520/11:43876864
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Sturgeon sperm; Nifedipine; K+-Ca2+ antagonist; EGTA; Ca2+ signalling; Asymmetric flagellar beating (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Alavi, Sayyed Mohammad Hadi
- Gela, David
- Linhart, Otomar
- Rodina, Marek
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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http://bibframe.org/vocab/doi
| - 10.1016/j.cbpa.2011.05.026
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http://localhost/t...ganizacniJednotka
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is http://linked.open...avai/riv/vysledek
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