About: In vitro effects of Bisphenol A on sperm motility characteristics in Perca fluviatilis L. (Percidae; Teleostei)

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Description	P>In the present study, the effects of Bisphenol A (BPA) on sperm motility and velocity were investigated in perch (Perca fluviatilis L.). Sperm of five mature males was separately collected in a syringe. The sperm samples were diluted in an immobilizing solution containing 180 mm NaCl, 2.7 mm KCl, 1.4 mm CaCl2, 2.4 mm NaHCO3, pH 8.0 (340 mOsmol kg-1) at ratio 1 : 50 (sperm : IS). The sperm motility was triggered in an activation solution composed of 50 mm NaCl, 20 mm Tris, pH 8.5 containing different concentrations of BPA (2, 1.5, 1.25, 1.0, 0.5, 0.25, 0.125 and 0.0 mm) at ratio 1 : 50 (sperm : AS). At 15 s post-activation, sperm motility and velocity decreased significantly in activation medium containing 1.5 and 1 mm BPA, respectively. The motility of sperm was totally inhibited in 2 mm of BPA. Sperm motility duration decreased with increasing BPA concentrations. In the control, 25.3% of spermatozoa were still motile at 90 s post-activation, while only 1.3% was in 0.12 mm. However, sperm velocit P>In the present study, the effects of Bisphenol A (BPA) on sperm motility and velocity were investigated in perch (Perca fluviatilis L.). Sperm of five mature males was separately collected in a syringe. The sperm samples were diluted in an immobilizing solution containing 180 mm NaCl, 2.7 mm KCl, 1.4 mm CaCl2, 2.4 mm NaHCO3, pH 8.0 (340 mOsmol kg-1) at ratio 1 : 50 (sperm : IS). The sperm motility was triggered in an activation solution composed of 50 mm NaCl, 20 mm Tris, pH 8.5 containing different concentrations of BPA (2, 1.5, 1.25, 1.0, 0.5, 0.25, 0.125 and 0.0 mm) at ratio 1 : 50 (sperm : AS). At 15 s post-activation, sperm motility and velocity decreased significantly in activation medium containing 1.5 and 1 mm BPA, respectively. The motility of sperm was totally inhibited in 2 mm of BPA. Sperm motility duration decreased with increasing BPA concentrations. In the control, 25.3% of spermatozoa were still motile at 90 s post-activation, while only 1.3% was in 0.12 mm. However, sperm velocit (en)
Title	In vitro effects of Bisphenol A on sperm motility characteristics in Perca fluviatilis L. (Percidae; Teleostei) In vitro effects of Bisphenol A on sperm motility characteristics in Perca fluviatilis L. (Percidae; Teleostei) (en)
skos:prefLabel	In vitro effects of Bisphenol A on sperm motility characteristics in Perca fluviatilis L. (Percidae; Teleostei) In vitro effects of Bisphenol A on sperm motility characteristics in Perca fluviatilis L. (Percidae; Teleostei) (en)
skos:notation	RIV/60076658:12520/10:00011434!RIV11-GA0-12520___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(ED2.1.00/01.0024), P(GA523/09/1793), P(IAA608030801), P(QH71305), S
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Alavi, Sayyed Mohammad Hadi Policar, Tomáš Linhart, Otomar Rodina, Marek Hatef, Azadeh
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Fakulta rybářství a ochrany vod
http://linked.open...dnocenehoVysledku	263292
http://linked.open...ai/riv/idVysledku	RIV/60076658:12520/10:00011434
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Medaka Oryzias-Latipes; Term Exposure; Semen; Quality; Xenoestrogens; Nonylphenol; Tributyltin; Disruption; Expression; Chemicals (en)
http://linked.open.../riv/klicoveSlovo	Expression Disruption Tributyltin Quality Xenoestrogens Medaka Oryzias-Latipes Nonylphenol Semen Term Exposure Chemicals
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[52DEBC101198]
http://linked.open...i/riv/nazevZdroje	Journal of Applied Ichthyology
http://linked.open...in/vavai/riv/obor	GL
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Effect of endocrine disruptors on reproductive parameters and expression of selected genes in mouse gonads. South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Diversity of Bioenergetics Pathways, Membrane Functions, Signaling Mechanisms and Proteomics of Cryopreserved Sperm of Evolutionary Different Fish Species Development of new methods of rearing selected promising species for aquaculture using non-traditional technologies.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	26
http://linked.open...iv/tvurceVysledku	Alavi, Sayyed Mohammad Hadi Linhart, Otomar Rodina, Marek Policar, Tomáš Hatef, Azadeh Linhartová, Z.
http://linked.open...ain/vavai/riv/wos	000281894400013
issn	0175-8659
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	12520
is http://linked.open...avai/riv/vysledek of	In vitro effects of Bisphenol A on sperm motility characteristics in Perca fluviatilis L. (Percidae; Teleostei)

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