| Attributes | Values |
|---|
| rdf:type
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| rdfs:seeAlso
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| Description
| - Post-thaw motility rate, curvilinear velocity (VCL), and fertilizing ability of carp spermatozoa can be improved by short-term treatment with moderately hypotonic media prior to freezing. Before cryopreservation, carp sperm samples were treated with NaCl solutions of differing osmolalities, ranging from 100 to 300 mOsm kg(-1) for 10 s, after which final osmolality was adjusted to 300 mOsm kg(-1). The resulting sperm suspension was diluted 1:1 with cryoprotective medium and frozen using conventional techniques. Control samples were treated in the same way, without the pre-dilution step. Post-thaw motility rate in samples pretreated with 200 mOsm kg(1) NaCl was significantly higher (44 +/- 10%) than in controls (21 +/- 15%) and samples pretreated with 100 mOsm kg(-1) (25 +/- 15%) and 300 mOsm kg(-1) (25 +/- 12%) NaCl. Significantly higher mean VCL were observed in samples pretreated with 100, 150, and 200 mOsm kg(-1) (119 +/- 24, 118 +/- 22, and 115 +/- 32 mu m s(-1), respectively) compared to controls (92 27 mu m s-1). Fertilization rate of frozen-thawed sperm treated with 200 mOsm kg(-1) solution of 2 M NaCl was significantly higher (25 +/- 18%) than that of sperm treated with 300 mOsm kg(-1) NaCl solutions (12 +/- 7%) and the control (9 +/- 6%).
- Post-thaw motility rate, curvilinear velocity (VCL), and fertilizing ability of carp spermatozoa can be improved by short-term treatment with moderately hypotonic media prior to freezing. Before cryopreservation, carp sperm samples were treated with NaCl solutions of differing osmolalities, ranging from 100 to 300 mOsm kg(-1) for 10 s, after which final osmolality was adjusted to 300 mOsm kg(-1). The resulting sperm suspension was diluted 1:1 with cryoprotective medium and frozen using conventional techniques. Control samples were treated in the same way, without the pre-dilution step. Post-thaw motility rate in samples pretreated with 200 mOsm kg(1) NaCl was significantly higher (44 +/- 10%) than in controls (21 +/- 15%) and samples pretreated with 100 mOsm kg(-1) (25 +/- 15%) and 300 mOsm kg(-1) (25 +/- 12%) NaCl. Significantly higher mean VCL were observed in samples pretreated with 100, 150, and 200 mOsm kg(-1) (119 +/- 24, 118 +/- 22, and 115 +/- 32 mu m s(-1), respectively) compared to controls (92 27 mu m s-1). Fertilization rate of frozen-thawed sperm treated with 200 mOsm kg(-1) solution of 2 M NaCl was significantly higher (25 +/- 18%) than that of sperm treated with 300 mOsm kg(-1) NaCl solutions (12 +/- 7%) and the control (9 +/- 6%). (en)
|
| Title
| - Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa
- Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa (en)
|
| skos:prefLabel
| - Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa
- Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa (en)
|
| skos:notation
| - RIV/60076658:12520/13:43885204!RIV14-MZE-12520___
|
| http://linked.open...avai/predkladatel
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(ED2.1.00/01.0024), P(GAP502/11/0090), P(IAA608030801), P(LC06073), P(ME10015), P(QH82119), S
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12520/13:43885204
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Cryoresistance; Hypotonic treatment; Carp sperm (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Dzyuba, Boris
- Gela, David
- Linhart, Otomar
- Rodina, Marek
- Cosson, Jacky
- Shaliutina, Anna
- Bondarenko, Volodymyr
- Bondarenko, Olga
- Yamaner, Gunes
|
| http://linked.open...ain/vavai/riv/wos
| |
| issn
| |
| number of pages
| |
| http://bibframe.org/vocab/doi
| - 10.1016/j.cryobiol.2012.12.003
|
| http://localhost/t...ganizacniJednotka
| |
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