About: Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces)

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About: Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces) Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Sturgeon and paddlefish (Acipenseriformes) are a unique and commercially valuable group of ancient fishes. In this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish. Protein profiles of spermatozoa were determined by 2-DE electrophoresis and by MALDI-TOF/MS. Among the 23 protein spots selected, 14 were identified as isoforms of enolase B present in all species, but with different isoelectric points or molecular mass. The glycerol-3-phosphate dehydrogenase was detected exclusively in P. spathula. Phosphoglycerate kinase was detected only in A. ruthenus and H. huso, and 3 additional proteins (fructose bisphosphate aldolase A-2, glycogen phosphorylase type IV and glyceraldehyde-3-phosphate dehydrogenase) were found exclusively in A. gueldenstaedtii and H. huso. This study points to the application of proteomics for differential characterization and comparative studies of acipenseriform species at the molecular level. Sturgeon and paddlefish (Acipenseriformes) are a unique and commercially valuable group of ancient fishes. In this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish. Protein profiles of spermatozoa were determined by 2-DE electrophoresis and by MALDI-TOF/MS. Among the 23 protein spots selected, 14 were identified as isoforms of enolase B present in all species, but with different isoelectric points or molecular mass. The glycerol-3-phosphate dehydrogenase was detected exclusively in P. spathula. Phosphoglycerate kinase was detected only in A. ruthenus and H. huso, and 3 additional proteins (fructose bisphosphate aldolase A-2, glycogen phosphorylase type IV and glyceraldehyde-3-phosphate dehydrogenase) were found exclusively in A. gueldenstaedtii and H. huso. This study points to the application of proteomics for differential characterization and comparative studies of acipenseriform species at the molecular level. (en)
Title	Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces) Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces) (en)
skos:prefLabel	Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces) Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces) (en)
skos:notation	RIV/60076658:12520/10:00011455!RIV11-MSM-12520___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(ED2.1.00/01.0024), P(IAA608030801), P(LC06073), P(LC07017), P(ME10015), P(QH82119), P(QH92308), S, Z(AV0Z50200510), Z(MSM6007665809)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Linhart, Otomar Hulák, Martin Rodina, Marek Li, Ping Li, Zhihua
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Fakulta rybářství a ochrany vod
http://linked.open...dnocenehoVysledku	251330
http://linked.open...ai/riv/idVysledku	RIV/60076658:12520/10:00011455
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Endangered species; Sperm; Comparative proteomics; MALDI-TOF/MS (en)
http://linked.open.../riv/klicoveSlovo	Endangered species Sperm Comparative proteomics MALDI-TOF/MS
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C80514AC026F]
http://linked.open...i/riv/nazevZdroje	Comparative Biochemistry and Physiology D-Genomics & Proteomics
http://linked.open...in/vavai/riv/obor	GL
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Diversity of Bioenergetics Pathways, Membrane Functions, Signaling Mechanisms and Proteomics of Cryopreserved Sperm of Evolutionary Different Fish Species Biodiversity Research Center Application of Paddlefish Sex-reversal and Proteomics in Its Cryopreserved Sperm for Caviar Production Research of sperm and embryos cryopreservation Oncoproteomics Centre Genetic diversity of sterlet (Acipenser ruthenus): implication for in situ conservation and protection of biodiversity
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	5D
http://linked.open...iv/tvurceVysledku	Hulák, Martin Li, Ping Li, Zhihua Linhart, Otomar Rodina, Marek Šulc, M.
http://linked.open...ain/vavai/riv/wos	000286303400008
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Biologické, enviromentální a chovatelské aspekty v rybářství
issn	1744-117X
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	12520
is http://linked.open...avai/riv/vysledek of	Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces) Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces) Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces)

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