About: Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa

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About: Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S0009279713000070
Description	The effects of vinclozolin (VIN), an anti-androgenic fungicide, on quality, oxidative stress, DNA integrity, and ATP level of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were incubated with different concentrations of vinclozolin (0.5, 2, 10, 15, 20 and 50 mu/l) for 2 h. A dose-dependent reduction in spermatozoa motility and velocity was observed at concentrations of 2-50 mu g/l. A dramatic increase in DNA fragmentation was recorded at concentrations 10 mu/l and above. After 2 h exposure at higher test concentrations (10-50 mu/l), oxidative stress was apparent, as reflected by significantly higher levels of protein and lipid oxidation and significantly greater superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of VIN. The results demonstrated that VIN can induce reactive oxygen species stress in fish spermatozoa, which could impair the sperm quality, DNA integrity, ATP content, and the antioxidant defense system. The effects of vinclozolin (VIN), an anti-androgenic fungicide, on quality, oxidative stress, DNA integrity, and ATP level of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were incubated with different concentrations of vinclozolin (0.5, 2, 10, 15, 20 and 50 mu/l) for 2 h. A dose-dependent reduction in spermatozoa motility and velocity was observed at concentrations of 2-50 mu g/l. A dramatic increase in DNA fragmentation was recorded at concentrations 10 mu/l and above. After 2 h exposure at higher test concentrations (10-50 mu/l), oxidative stress was apparent, as reflected by significantly higher levels of protein and lipid oxidation and significantly greater superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of VIN. The results demonstrated that VIN can induce reactive oxygen species stress in fish spermatozoa, which could impair the sperm quality, DNA integrity, ATP content, and the antioxidant defense system. (en)
Title	Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa (en)
skos:prefLabel	Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa Influence of environmentally relevant concentrations of vinclozolin on quality, DNA integrity, and antioxidant responses of sterlet Acipenser ruthenus spermatozoa (en)
skos:notation	RIV/60076658:12520/13:43885223!RIV14-MZE-12520___
http://linked.open...avai/predkladatel	Fakulta rybářství a ochrany vod
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(ED2.1.00/01.0024), P(GAP502/11/0090), P(IAA608030801), P(ME10015), P(QH82119), S
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Linhartová, Pavla Hulák, Martin Shaliutina, Anna Gazo, Ievgenia
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Fakulta rybářství a ochrany vod
http://linked.open...dnocenehoVysledku	79750
http://linked.open...ai/riv/idVysledku	RIV/60076658:12520/13:43885223
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Sturgeon; ATP; Oxidative stress; DNA damage; Spermatozoa motility (en)
http://linked.open.../riv/klicoveSlovo	Spermatozoa motility ATP Sturgeon DNA damage Oxidative stress
http://linked.open...odStatuVydavatele	IE - Irsko
http://linked.open...ontrolniKodProRIV	[89D34D18AEE8]
http://linked.open...i/riv/nazevZdroje	Chemico-biological interactions
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Maturation and Ageing of Fish Spermatozoa: A Comparative Study Between Teleostean and Chondrostean Fish Species as Taxonomically Distant Models. South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Diversity of Bioenergetics Pathways, Membrane Functions, Signaling Mechanisms and Proteomics of Cryopreserved Sperm of Evolutionary Different Fish Species Application of Paddlefish Sex-reversal and Proteomics in Its Cryopreserved Sperm for Caviar Production Research of sperm and embryos cryopreservation
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	203
http://linked.open...iv/tvurceVysledku	Hulák, Martin Shaliutina, Anna Gazo, Ievgenia Linhartová, Pavla
http://linked.open...ain/vavai/riv/wos	000319235100001
issn	0009-2797
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.cbi.2013.01.004
http://localhost/t...ganizacniJednotka	12520

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