About: γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis

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About: γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	While γ-tubulin 1 is ubiquitous, γ-tubulin 2 is known to be not able to substitute for γ-tubulin 1 deficiency in blastocysts. This research article presents our contribution to deciphering of γ-tubulin 2 role. We have found that exogenous γ-tubulin 2 is targeted to centrosomes and interacts with γ-tubulin complex proteins 2 and 4. Depletion of γ-tubulin 1 by RNAi in U2Os cells causes impaired microtubule nucleation and metaphase arrest. Wild-type phenotype in γ-tubulin 1-depleted cells is restored by expression of exogenous mouse and human γ-tubulin 2. Using RT-qPCR and 2D-PAGE, we have found that Tubg2 expression is dramatically reduced in mouse blastocysts. This indicates that γ-tubulin 2 cannot rescue γ-tubulin 1 deficiency in knock-out blastocysts. The obtained data suggest that γ-tubulin 2 is able to nucleate microtubules and subtitute for γ-tubulin 1. We propose that mammalian γ-tubulins are functionally redundant with respect to the nucleation activity of microtubules. While γ-tubulin 1 is ubiquitous, γ-tubulin 2 is known to be not able to substitute for γ-tubulin 1 deficiency in blastocysts. This research article presents our contribution to deciphering of γ-tubulin 2 role. We have found that exogenous γ-tubulin 2 is targeted to centrosomes and interacts with γ-tubulin complex proteins 2 and 4. Depletion of γ-tubulin 1 by RNAi in U2Os cells causes impaired microtubule nucleation and metaphase arrest. Wild-type phenotype in γ-tubulin 1-depleted cells is restored by expression of exogenous mouse and human γ-tubulin 2. Using RT-qPCR and 2D-PAGE, we have found that Tubg2 expression is dramatically reduced in mouse blastocysts. This indicates that γ-tubulin 2 cannot rescue γ-tubulin 1 deficiency in knock-out blastocysts. The obtained data suggest that γ-tubulin 2 is able to nucleate microtubules and subtitute for γ-tubulin 1. We propose that mammalian γ-tubulins are functionally redundant with respect to the nucleation activity of microtubules. (en)
Title	γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis (en)
skos:prefLabel	γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis (en)
skos:notation	RIV/68378050:_____/12:00375928!RIV13-GA0-68378050
http://linked.open...avai/predkladatel	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0506), P(GA204/09/1777), P(GAP302/10/1701), P(GD204/09/H084), P(KAN200520701), P(LC545), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Černohorská, Markéta Vinopal, Stanislav Dráber, Pavel Dráberová, Eduarda Sulimenko, Tetyana Flemr, Matyáš Sulimenko, Vadym Vosecká, Věra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	183166
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/12:00375928
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	γ-tubulin 2; nucleation; embryogenesis (en)
http://linked.open.../riv/klicoveSlovo	γ-tubulin 2 nucleation embryogenesis
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[DE28A5B4810F]
http://linked.open...i/riv/nazevZdroje	PLoS ONE
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional Organisation of the Cell Center of Molecular and Cellular Immunology Molecular biology of a differentiating cell Nano-PCR - ultrasensitive test for detection of specific proteins in body fluids Analysis of signaling assemblies containing gamma-tubulin Analysis of functional differences between gamma-tubulins
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Dráber, Pavel Dráberová, Eduarda Sulimenko, Tetyana Vinopal, Stanislav Flemr, Matyáš Sulimenko, Vadym Vosecká, Věra Černohorská, Markéta
http://linked.open...ain/vavai/riv/wos	000301123400112
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	1932-6203
number of pages	14 (xsd:int)
http://bibframe.org/vocab/doi	10.1371/journal.pone.0029919
is http://linked.open...avai/riv/vysledek of	γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis

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