About: High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Mouse NKR-P1C(B6) receptor corresponding to NK1.1 alloantigen is one of the most widespread surfacemarkers of mouse NK and NKT cells in C57BL/6 mice detected by monoclonal antibody PK136. Althoughfunctional studies revealed the ability of this receptor to activate both natural killing and production ofcytokines upon antibody crosslinking, the ligand for NKR-P1C(B6) remains unknown. In order to initiate ligand identification, structural studies, and epitope mapping experiments, we developed a simple and efficient expression and purification protocol allowing to produce large amounts of pure soluble monomeric mouse NKR-P1C(B6). Our protein encompassed approximately half of the stalk region and the entire C-terminal globular ligand binding domain Mouse NKR-P1C(B6) receptor corresponding to NK1.1 alloantigen is one of the most widespread surfacemarkers of mouse NK and NKT cells in C57BL/6 mice detected by monoclonal antibody PK136. Althoughfunctional studies revealed the ability of this receptor to activate both natural killing and production ofcytokines upon antibody crosslinking, the ligand for NKR-P1C(B6) remains unknown. In order to initiate ligand identification, structural studies, and epitope mapping experiments, we developed a simple and efficient expression and purification protocol allowing to produce large amounts of pure soluble monomeric mouse NKR-P1C(B6). Our protein encompassed approximately half of the stalk region and the entire C-terminal globular ligand binding domain (en)
Title	High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli (en)
skos:prefLabel	High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli (en)
skos:notation	RIV/61388971:_____/11:00369717!RIV12-AV0-61388971
http://linked.open...avai/predkladatel	Mikrobiologický ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(1M0505), P(GA303/09/0477), P(GAP207/10/1040), P(GD305/09/H008), P(IAA500200620), P(LC07017), P(LC545), S, Z(AV0Z50200510), Z(MSM0021620808)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Bezouška, Karel Kavan, Daniel Novák, Petr Rozbeský, Daniel Vaněk, Ondřej Chmelík, Josef
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	202069
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/11:00369717
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Natural killer cell; NKR-P1C receptor; NK1.1 antigen (en)
http://linked.open.../riv/klicoveSlovo	NK1.1 antigen NKR-P1C receptor Natural killer cell
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[42B1EBF70FE3]
http://linked.open...i/riv/nazevZdroje	Protein Expression and Purification
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Dynamics of CD161 antigen expression on cytotoxic cells and involved signaling pathways upon saccharide ligand interaction Center of targeted therapeutic drugs Preparation, biotransformation, and optimalization of compounds with antitumor and antimicrobial effects Functional Organisation of the Cell Oncoproteomics Centre Glycomic and proteomic analysis of the molecular mechanisms of elimination of clinically relevant tumors by killer cells of the immune system Analysis of protein complexes of killer cell receptors using chemical cross-linking, surface labeling, isotope exchange, mass spectrometry and NMR.
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	77
http://linked.open...iv/tvurceVysledku	Bezouška, Karel Kavan, Daniel Novák, Petr Vaněk, Ondřej Chmelík, Josef Rozbeský, Daniel
http://linked.open...ain/vavai/riv/wos	000288591900008
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Molekulárně biologické, genetické a epigenetické aspekty vzniku a rozvoje modelových tumorů dospělého věku. Význam pro epidemiologii, časnou diagnostiku a léčbu
issn	1046-5928
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.pep.2011.01.013
is http://linked.open...avai/riv/vysledek of	High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 44 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software