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rdf:type
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Description
| - Mouse NKR-P1C(B6) receptor corresponding to NK1.1 alloantigen is one of the most widespread surfacemarkers of mouse NK and NKT cells in C57BL/6 mice detected by monoclonal antibody PK136. Althoughfunctional studies revealed the ability of this receptor to activate both natural killing and production ofcytokines upon antibody crosslinking, the ligand for NKR-P1C(B6) remains unknown. In order to initiate ligand identification, structural studies, and epitope mapping experiments, we developed a simple and efficient expression and purification protocol allowing to produce large amounts of pure soluble monomeric mouse NKR-P1C(B6). Our protein encompassed approximately half of the stalk region and the entire C-terminal globular ligand binding domain
- Mouse NKR-P1C(B6) receptor corresponding to NK1.1 alloantigen is one of the most widespread surfacemarkers of mouse NK and NKT cells in C57BL/6 mice detected by monoclonal antibody PK136. Althoughfunctional studies revealed the ability of this receptor to activate both natural killing and production ofcytokines upon antibody crosslinking, the ligand for NKR-P1C(B6) remains unknown. In order to initiate ligand identification, structural studies, and epitope mapping experiments, we developed a simple and efficient expression and purification protocol allowing to produce large amounts of pure soluble monomeric mouse NKR-P1C(B6). Our protein encompassed approximately half of the stalk region and the entire C-terminal globular ligand binding domain (en)
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Title
| - High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli
- High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli (en)
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skos:prefLabel
| - High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli
- High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli (en)
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skos:notation
| - RIV/61388971:_____/11:00369717!RIV12-AV0-61388971
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http://linked.open...avai/predkladatel
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(1M0505), P(GA303/09/0477), P(GAP207/10/1040), P(GD305/09/H008), P(IAA500200620), P(LC07017), P(LC545), S, Z(AV0Z50200510), Z(MSM0021620808)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/11:00369717
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Natural killer cell; NKR-P1C receptor; NK1.1 antigen (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Protein Expression and Purification
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Bezouška, Karel
- Kavan, Daniel
- Novák, Petr
- Vaněk, Ondřej
- Chmelík, Josef
- Rozbeský, Daniel
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://bibframe.org/vocab/doi
| - 10.1016/j.pep.2011.01.013
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is http://linked.open...avai/riv/vysledek
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