About: Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Production of soluble forms of cell surface receptors suitable for biochemical and structural studies is critical in modern membrane biology and biochemistry. We have been investigating the soluble forms of the earliest activation antigen of human leukocytes CD69. This receptor is naturally expressed at the cell surface as type II homodimeric membrane protein. However, the elements necessary to prepare the soluble recombinant CD69 suitable for structural studies have been a matter of controversy. We describe here the physical, biochemical and in vivo characteristics of a highly stable soluble form of CD69 obtained by bacterial expression of an optimized extracellular segment corresponding to amino acids Gly70 to Lys199. This optimized protein construct has been derived from the one used previously for CD69 crystallization by its further optimalization with regard to protein stability, solubility, and easy crystallization under conditions promoting ligand binding. Production of soluble forms of cell surface receptors suitable for biochemical and structural studies is critical in modern membrane biology and biochemistry. We have been investigating the soluble forms of the earliest activation antigen of human leukocytes CD69. This receptor is naturally expressed at the cell surface as type II homodimeric membrane protein. However, the elements necessary to prepare the soluble recombinant CD69 suitable for structural studies have been a matter of controversy. We describe here the physical, biochemical and in vivo characteristics of a highly stable soluble form of CD69 obtained by bacterial expression of an optimized extracellular segment corresponding to amino acids Gly70 to Lys199. This optimized protein construct has been derived from the one used previously for CD69 crystallization by its further optimalization with regard to protein stability, solubility, and easy crystallization under conditions promoting ligand binding. (en)
Title	Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice (en)
skos:prefLabel	Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice (en)
skos:notation	RIV/00216224:14310/08:00026574!RIV11-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0505), P(IAA500200509), P(IAA5020403), P(LC06030), P(LC07017), P(LC545), Z(AV0Z40500505), Z(AV0Z50200510), Z(AV0Z50520514), Z(MSM0021620808), Z(MSM0021620835), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	275
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Hofbauerová, Kateřina Gorčík, Lukáš Bezouška, Karel Brynda, Jiří Sklenář, Vladimír Kadeřávek, Pavel Žídek, Lukáš Chmelík, Josef Kubíčková, Monika
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	395826
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/08:00026574
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	C-type lectin leukocyte activation plasma clearance refolding stability (en)
http://linked.open.../riv/klicoveSlovo	C-type lectin leukocyte activation plasma clearance refolding stability
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[FE4EAD09121F]
http://linked.open...i/riv/nazevZdroje	the FEBS Journal
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	9 (xsd:int)
http://linked.open...cetTvurcuVysledku	20 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of targeted therapeutic drugs Biomolecular centre Functional Organisation of the Cell Oncoproteomics Centre Immune recognition and cell signaling in cancer modulation by bylcoconjugates Tumor stress-induced proteins as target structures for cytotoxic cells
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	2008
http://linked.open...iv/tvurceVysledku	Bezouška, Karel Brynda, Jiří Dohnálek, Jan Hofbauerová, Kateřina Hudeček, Jiří Kadeřávek, Pavel Kavan, Daniel Novák, Petr Sklenář, Vladimír Vannucci, Luca Vaněk, Ondřej Žídek, Lukáš Pompach, Petr Chmelík, Josef Borovičková, Ivana Kubíčková, Monika Kumar, Vinay Gorčík, Lukáš Koleno, Petr Kopecký, Vladimír, Jr
http://linked.open...ain/vavai/riv/wos	000260348600011
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Microorganisms in Research and Biotechnology Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů Molekulárně biologické, genetické a epigenetické aspekty vzniku a rozvoje modelových tumorů dospělého věku. Význam pro epidemiologii, časnou diagnostiku a léčbu Fyzika molekulárních, makromolekulárních a biologických systémů Proteiny v metabolismu a při interakci organismů s prostředím
issn	1742-464X
number of pages	18 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemicsl stability display prolonged circulation and remain intact in the blood of mice

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