The project deals with the analysis and clarification of mechanisms of adverse effects caused by human exposure to xenobiotics in the ambient air with special attention paid to carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) bound onto respirable dust particles. The changes in the gene expression and various biomarkers will be identified in model systems in vitro and in lymphocytes of subjects exposed to polluted air. The limit values for benzo(a)pyrene (B[a]P) as a major c-PAH representative and dust particles PM2.5 will be suggested by comparison of the results for various localities and sampling seasons. These limit values might be regarded as safe from the point of view of the biological effect studied. (en)
S použitím nových metod toxikogenomiky a molekulární epidemiologie definovat kritické koncentrace vybraných toxických látek (B[a]P, PM2.5) ve vnějším ovzduší, které již vyvolávají významné biologické změny v lidském organismu.