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  • Po SDS-PAGE elektroforéze jsou proteiny eluovány, renaturovány a použity pro retardační analýzu se značenými oligonukleotidy odpovídajícími telomerové sekvenci. Tato metoda je účinná k obnovení sekvenčně-specifických vazebných schopností kandidátních telomer-vazebných proteinů. (cs)
  • After sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), proteins are eluted, renatured and used for retardation analysis with labelled oligonucleotides corresponding to telomeric sequences. This method is efficient to recover sequence-specific DNA-binding abilities of putative telomere-binding proteins.
  • After sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), proteins are eluted, renatured and used for retardation analysis with labelled oligonucleotides corresponding to telomeric sequences. This method is efficient to recover sequence-specific DNA-binding abilities of putative telomere-binding proteins. (en)
Title
  • Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis
  • Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis (en)
  • Renaturace telomer-vazebných proteinů po frakcionaci pomocí SDS-PAGE (cs)
skos:prefLabel
  • Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis
  • Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis (en)
  • Renaturace telomer-vazebných proteinů po frakcionaci pomocí SDS-PAGE (cs)
skos:notation
  • RIV/68081707:_____/07:00083867!RIV08-AV0-68081707
http://linked.open.../vavai/riv/strany
  • 317;320
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA521/05/0055), P(IAA600040505), P(LC06004), Z(AV0Z50040507), Z(AV0Z50040702)
http://linked.open...iv/cisloPeriodika
  • 7
http://linked.open...vai/riv/dodaniDat
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http://linked.open...iv/duvernostUdaju
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  • 447341
http://linked.open...ai/riv/idVysledku
  • RIV/68081707:_____/07:00083867
http://linked.open...riv/jazykVysledku
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  • renaturation; telomere-binding proteins; telomeres (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [1B76BE19AB83]
http://linked.open...i/riv/nazevZdroje
  • Plant, Soil and Environment
http://linked.open...in/vavai/riv/obor
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 53
http://linked.open...iv/tvurceVysledku
  • Rotková, Gabriela
http://linked.open...n/vavai/riv/zamer
issn
  • 1214-1178
number of pages
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