About: Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Paper presents a complete sample handling protocol, which allows processing of disulfide containing proteins at basic pH. We modified the standard SDS gel electrophoresis and protein digestion conditions by the addition of an oxidative agent, cystamine. Thismodification prevented disulfide scrambling, which we otherwise observed in the samples handled according to the general protocol. Lysozyme from hen egg was used as a model protein for the development of the method. Disulfide bonds were characterized in the following proteins - human leukocyte antigen CD69, murine leukocyte receptor NKR-P1A and glycosylated β-N-acetylhexosaminidases from Aspergillus oryzae and Penicillium oxalicum Paper presents a complete sample handling protocol, which allows processing of disulfide containing proteins at basic pH. We modified the standard SDS gel electrophoresis and protein digestion conditions by the addition of an oxidative agent, cystamine. Thismodification prevented disulfide scrambling, which we otherwise observed in the samples handled according to the general protocol. Lysozyme from hen egg was used as a model protein for the development of the method. Disulfide bonds were characterized in the following proteins - human leukocyte antigen CD69, murine leukocyte receptor NKR-P1A and glycosylated β-N-acetylhexosaminidases from Aspergillus oryzae and Penicillium oxalicum (en)
Title	Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds (en)
skos:prefLabel	Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds (en)
skos:notation	RIV/61388971:_____/09:00337531!RIV10-MSM-61388971
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA5020403), P(KJB400200501), P(KJB500200612), P(LC07017), P(LC545), Z(AV0Z50200510), Z(MSM0021620808), Z(MSM0021620835), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Bezouška, Karel Kumar, Vinay Kavan, Daniel Man, Petr Havlíček, Vladimír Novák, Petr Hofbauerová, Kateřina Pompach, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	327130
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/09:00337531
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	disulfide bond; cystamine; gel electrophoresis (en)
http://linked.open.../riv/klicoveSlovo	cystamine disulfide bond gel electrophoresis
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[8368F72B5D35]
http://linked.open...i/riv/nazevZdroje	Journal of Mass Spectrometry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Proteomic characterization of human natural killer cell membrane microdomains Functional Organisation of the Cell Oncoproteomics Centre Tumor stress-induced proteins as target structures for cytotoxic cells Determination of the Three-Dimensional Structure of Secreted Aspartic Proteases Using Chemical Cross-Linking and High Resolution Mass Spectrometry
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	44
http://linked.open...iv/tvurceVysledku	Bezouška, Karel Hofbauerová, Kateřina Kavan, Daniel Man, Petr Novák, Petr Havlíček, Vladimír Pompach, Petr Kumar, Vinay
http://linked.open...ain/vavai/riv/wos	000272150300004
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Molekulárně biologické, genetické a epigenetické aspekty vzniku a rozvoje modelových tumorů dospělého věku. Význam pro epidemiologii, časnou diagnostiku a léčbu Fyzika molekulárních, makromolekulárních a biologických systémů Modulation of signalling and regulatory pathways in normal and cancer cells
issn	1076-5174
number of pages	8 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds

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