About: Novel approaches for effective analysis and mapping of genomes in cultivated plants

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Projekt, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Projekt
rdfs:seeAlso	http://www.isvav.cz/projectDetail.do?rowId=GV521/96/K117
Description	This project proposes a novel and powerful approach for the efficient analysis and mapping of large eukaryotic genomes. Specifically, we propose the development of means for the construction of chromosome-specific DNA libraries, isolation of chromosome-specific DNA sequences and markers, and the development of chromosome paints for economically important cereals (Zea mays, Secale cereale, Hordeum vulgare and Triticum aestivum), legumes (Vicia faba, Pisum sativum, Medicago sativa), a coniferous tree (Picea abies), and a dioecious plant Melandrium album. The project is based on a multidisciplinary approach combining classical genetics (the use of isogenic and translocation lines) with flow cytogenetics (chromosome isolation and flow-sorting of single chromosome types), molecular techniques (DNA isolation, cloning and primed in situ DNA labelling). The ultimate goal will be to use chromosome-specific libraries, markers and paints to increase the density and accuracy of genetics (en) Projekt je zaměřen na strukturní analýzu a mapování genomů vyšších rostlin novými a účinnějšími metodickými postupy. Budou vyvinuty nebo zdokonaleny techniky pro konstrukci chromozómově specifických knihoven DNA, cílenou izolaci markerů z vybraných oblastí genomu a přípravu komplexních sond pro celé chromozómy, a to u ekonomicky významných druhů obilovin (Zea mays, Secale cereale, Hordeum vulgare and Triticum aestivum), leguminóz (Vicia faba, Pisum sativum, Medicago sativa), smrku ztepilého (Picea abies) a knotovky bílé (Melandrium album). Projekt je založen na mezioborovém přístupu, kombinujícím klasickou genetiku a cytogenetiku s průtokovou cytometrií (izolace chromozómů a separace jejich jednotlivých typů), molekulárními technikami (izolace, klonování a sekvenování DNA) a molekulární cytogenetikou (in situ hybridizace a chromosome painting). Konečným cílem je využití chromozómově specifických knihoven, markerů a komplexních sond pro zahuštění a zpřesnění genetických map, izolaci a lokalizaci
Title	Novel approaches for effective analysis and mapping of genomes in cultivated plants (en) Nové metody pro efektivní studium a mapování genomů kulturních rostlin
skos:notation	GV521/96/K117
http://linked.open...avai/cep/aktivita	Comprehensive projects
http://linked.open...kovaStatniPodpora	http://linked.opendata.cz/resource/domain/vavai/projekt/GV521%2F96%2FK117/celkovaStatniPodpora
http://linked.open...ep/celkoveNaklady	http://linked.opendata.cz/resource/domain/vavai/projekt/GV521%2F96%2FK117/celkoveNaklady
http://linked.open...datumDodatniDoRIV	2002-11-19 (xsd:date)
http://linked.open...i/cep/druhSouteze	VS - Veřejná soutěž ve výzkumu a vývoji
http://linked.open...ep/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open.../cep/fazeProjektu	877074
http://linked.open...ai/cep/hlavniObor	GE - Šlechtění rostlin
http://linked.open...hodnoceniProjektu	U - Uspěl podle zadání, tj. byly splněny cíle a jeho předpokládané výsledky uvedené ve smlouvě / rozhodnutí o poskytnutí podpory
http://linked.open.../cep/klicovaSlova	Neuvedeno. (en)
http://linked.open...ep/partnetrHlavni	Ústav experimentální botaniky AV ČR, v.v.i.
http://linked.open...inujicichPrijemcu	0 (xsd:int)
http://linked.open...cep/pocetPrijemcu	1 (xsd:int)
http://linked.open...ocetSpoluPrijemcu	6 (xsd:int)
http://linked.open.../pocetVysledkuRIV	74 (xsd:int)
http://linked.open...enychVysledkuVRIV	74 (xsd:int)
http://linked.open...iciPoslednihoRoku	2002
http://linked.open...atUdajeProjZameru	2002
http://linked.open...usZobrazovaneFaze	DUU
http://linked.open...ai/cep/typPojektu	P - Projekt výzkumu a vývoje financovaný ze státního rozpočtu
http://linked.open.../cep/vedlejsiObor	EF - Botanika EI - Biotechnologie a bionika
http://linked.open...jektu+dodavatelem	Cíl komplexního (6ti letého) projektu, vypracování a zavedení nových metod analýzy genomu byl splněn. Průtoková cytometrie a třídění chromosomů pomůže dalšímu rozvoji genomiky v ČR. Významnou součástí projektu byla výchova studentů na jednotlivých pracov (cs)
http://linked.open...tniCyklusProjektu	ZBBBBKU
is http://linked.open...vavai/riv/projekt of	Localisation of DNA sequences on plant chromosomes using PRINS and C-PRINS. Karyological characterization of sugar beet gynogenetic lines cultured in vitro. Localization of male-specifically expressed MROS genes on Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes. Localization of Male-Specifically Expressed MROS Genes of Silene latifolia by PCR on Flow-Sorted Sex Chromosomes and Autosomes. Isolation of chromosomes from Picea abies and their analysis by flow cytometry The effect of a short heat treatment on the in vitro induced androgenesis in Silene latifolia ssp. alba N/A Cytogenetic variation in tissue culture-derived Hypericum perforatum L. plants and their seed progenies. Cytogenetic variability of in vitro regenerated Hypericum perforatum L. plants and their seed progenies. Localisation of GAA microsatellites on barley chromosomes by direct cycling-prins (C-Prins). Methylation of mitochondrial DNA in carrot (Daucus carota L.). Edentification of flow-sorted cereal chromosomes after cycling PRINS with microsatellite primers. Progress in plant flow cytogenetics. Vicia faba germination:synchronized cell growth and localization of nucleolin and alpha-tubulin. Vicia faba germination: Synchronized cell growth and localizaion of nucleolin and .alfa.-tubulin. Construction of plant BAC libraries. Flow Sorting of Mitotic Chromosomes in Common Wheat (Triticum aestivum L.). Identification and chromosomal localization of the monkey retrotransposon in Mesa sp. Fyzická lokalizace DNA sekvencí na rostlinných chromosomech metodou PRINS. Flow cytometry sorting and construction chromosome specific library of Norway spruce (Picea abies (L.) Karst) Rapid Identification and Determination of Purity of Flow-Sorted Plant Chromosomes Using C-PRINS. Seed germination in a tomato male-sterile mutant is resistant to osmotic, salt and low-temperature stresses. Reduced de-etiolation of hypocotyl growth in a tomato mutant is associated with hypersensitivity to, and high endogenous levels of, abscisic acid. Development and characterization of microsatellite markers from chromosome 1-specific DNA libraries of Vicia faba. A combined PRINS-FISH technique for simultaneous localisation of DNA sequences on plant chromosomes. Cells cycle synchronisation and chromosome isolation in barley (Hordeum vulgare). A Cytosin Technique for Spreading Plant Metaphases Suitable for Immunofluorescence Studies. Two new families of tandem repeats isolated from genus Vicia using genomic self-priming PCR. A Taxonomic Study of the Vaccinum Sect. Oxycoccus (Hill) W.D.J. Koch (Ericaceae) in the Czech Republic and Adjacement Territories. Application of an optical immersion-gel in a flow cytometer with horizontally oriented objective. Sorting of Plant Chromosomes. Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry. Chromosome sorting and PCR-based physical mapping in pea (Pisum sativum L.). PlantSat: a specialized database for plant satellite repeats. Isolation of chromosomes from Pisum sativum L. hairy roots and their analysis by flow cytometry. Flow cytometry, its application and potential for plant breeding. Nuclear DNA content and ploidy analyses in higher plants. Optimization of PRINS and C-PRINS for detection of telomeric sequences in Vicia faba. Plant genome size estimation by flow cytometry: inter-laboratory comparison. Cell cycle synchronization in plant root meristems. Flow Analysis and Sorting of Plant Chromosomes. Flow cytometric analysis and sorting of mitotic chromosomes in barley (Hordeum vulgare L.). Flow karyotyping and sorting of mitotic chromosomes of barley(Hordeum vulgare L.). Improved method for preparation of high-molecular-weight DNA from nuclei and chromosomes. Flow cytometric sorting of individual chromosomes in barley. Flow karyotyping ofNorway spruce (Picea abies L. Karst) Flow cytometry analysis and PCR with chromosome-specific primers of chromosome suspension of Norway spruce Morphological and physiological characteristics of plants issued from an interspecific hybridization of Cucumis sativus x Cucumis melo. Optimisation of C-PRINS for chromosome identification in barley and wheat. Evolutionary conservation of kinetochore protein sequences in plants. Effects of free and chelated iron on in vitro androgenesis in barley and wheat Limited Genome Size Variation in Sesleria albicans. Konstrukce DNA knihoven dlouhých insertů klonovaných ve vektoru BaC. Konstrukce částečné chomozomově-specifické knihovny optimalizace metody Nuclear Gamma-Tubulin during Acentriolar Plant Mitosis. Nuclear .tau.-Tubulin during Acentriolar Plant Mitosis. Mitotic index analysis with respect to moon phase Retroelements. Construction of recombinant DNA libraries from flow-sorted Vicia faba L. chromosomes. Construction of chromosome-specific DNA libraries covering the whole genome of field bean (Vicia faba L). Transgenesis - A scheme for improving sugar beet productivity. N/A 7 SL RNA palymorphism in hop (Humulus lupulus L.). Chromatin fragmentation associated with apoptotic changes in tobacco cells exposed to cold stress. Effectiveness of three micropropagation techniques to dissociate cytochimeras in Musa spp. Development of flow cytogenetics for hexaploid wheat (Triticum aestivum L.) Dioecious Plants. A Key to the Early Events of Sex Chromosome Evolution. In vivo metabolism of 2,6,9-trisubstituted purine-derived cyclin-dependent kinase inhibitor bohemine in mice: Glucosidation as the principal metabolic route. In vitro haploid zygotic embryogenesis due to pollination with maize pollen and induced in vitro androgenesis in Czech wheat breeding genotypes.

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