About: Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry.

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About: Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The analysis of wheat genome could be simplified by its separation into individual chromosome and/or chromosome arms. The aim of this study was to develop an improved procedure for preparation of chromosome suspensions and to evaluate the potential of flow cytometry for chromosome sorting in wheat. Suspensions of intact chromosomes were prepared by mechanical homogenisation of synchronized root tip meristems after a mild formaldehyde fixation. The analysis of DAPI-staining chromosome suspensions resulted in flow karyotypes containing three composite peaks representing groups of chromosomes. In addition, a peak that represented chromosome 3B was well resolved. This chromosome could be sorted at high purity. Sorting of other chromosomes was not possible.The work is in progress to assess the suitability of various chromosome stocks to overcome this problem. For instance using chromosome translocation lines it was possible to sort chromosome 5BL/7BL. Furthermore, some ditelosomic lines were found suitabl The analysis of wheat genome could be simplified by its separation into individual chromosome and/or chromosome arms. The aim of this study was to develop an improved procedure for preparation of chromosome suspensions and to evaluate the potential of flow cytometry for chromosome sorting in wheat. Suspensions of intact chromosomes were prepared by mechanical homogenisation of synchronized root tip meristems after a mild formaldehyde fixation. The analysis of DAPI-staining chromosome suspensions resulted in flow karyotypes containing three composite peaks representing groups of chromosomes. In addition, a peak that represented chromosome 3B was well resolved. This chromosome could be sorted at high purity. Sorting of other chromosomes was not possible.The work is in progress to assess the suitability of various chromosome stocks to overcome this problem. For instance using chromosome translocation lines it was possible to sort chromosome 5BL/7BL. Furthermore, some ditelosomic lines were found suitabl (en)
Title	Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry. Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry. (en)
skos:prefLabel	Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry. Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry. (en)
skos:notation	RIV/61989592:15310/01:00001274!RIV/2002/GA0/153102/N
http://linked.open.../vavai/riv/strany	531-536
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GV521/96/K117)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Doležel, Jaroslav Kubaláková, Marie Číhalíková, Jarmila
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	696430
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/01:00001274
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	flow cytometry; common wheat (Triticum aestivum L.); sorting of mitotic chromosomes; (en)
http://linked.open.../riv/klicoveSlovo	flow cytometry common wheat (Triticum aestivum L.) sorting of mitotic chromosomes
http://linked.open...ontrolniKodProRIV	[FD87A0167E3B]
http://linked.open...i/riv/mistoVydani	Dordrecht
http://linked.open...vEdiceCisloSvazku	N
http://linked.open...i/riv/nazevZdroje	Bedö, Z., Láng, L. (eds.): Wheat in a Global Environment
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Novel approaches for effective analysis and mapping of genomes in cultivated plants
http://linked.open...UplatneniVysledku	2001
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Kubaláková, Marie Číhalíková, Jarmila Šimková, Hana Vrána, J. Lysák, M. A.
number of pages	787 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Kluwer
https://schema.org/isbn	0-7923-6722-7
http://localhost/t...ganizacniJednotka	15310

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