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Description
| - Účinek krátkodobého tepelného působení v kombinaci s různými kultivačními médii na indukovanou androgenezi in vitro byl studován u Silene latifloia ssp. alba. Krátkodobé teplotní působení (33 °C, a 37 °C) bylo aplikováno po dobu 1, 3, či 5 dní. U všech použitých médií byla zaznamenána nejlepší androgenní odezva u kontrolní varianty 25 °C a po jednodenním působení v 33 °C. Všechna další působení snižovala frekvenci androgeneze. Nejlepších výsledků bylo mezi testovanými médii dosaženo při použití BMS media s 0,5 mg/l BAP a sacharózou. Mezi odvozenými regeneranty se vyskytovaly jak zelené, tak albikátní či chimerické rostliny. Pomocí flow cytometrie byly mezi 110 měřenými rostlinami identifikovány mixoploidy (n+2n a 2n+4n) a dihaploidy. (cs)
- The effect of a short heat treatment in combination with different culture medium composition on the efficiency of in vitro induced androgenesis in Silene latifolia ssp. alba was studied. The heat shock treatments (33 °C, and 37 °C) was applied for 1, 3, and 5 d. Across all media compositions tested, the best androgenic response was observed at 25 °C and after a one-day treatment at 33 °C. All other treatments reduced androgenic response. Among different media compositions tested, the most satisfactory results were obtained on BMS medium supplemented with 6-benzylaminopurine (0.5 mg dm-3) and sucrose. The green, albino and chimeric, only female, plants were regenerated. Flow cytometry of 110 regenerants identified mixoploids (n+2n and 2n+4n) and dihaploids.
- The effect of a short heat treatment in combination with different culture medium composition on the efficiency of in vitro induced androgenesis in Silene latifolia ssp. alba was studied. The heat shock treatments (33 °C, and 37 °C) was applied for 1, 3, and 5 d. Across all media compositions tested, the best androgenic response was observed at 25 °C and after a one-day treatment at 33 °C. All other treatments reduced androgenic response. Among different media compositions tested, the most satisfactory results were obtained on BMS medium supplemented with 6-benzylaminopurine (0.5 mg dm-3) and sucrose. The green, albino and chimeric, only female, plants were regenerated. Flow cytometry of 110 regenerants identified mixoploids (n+2n and 2n+4n) and dihaploids. (en)
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Title
| - The effect of a short heat treatment on the in vitro induced androgenesis in Silene latifolia ssp. alba
- The effect of a short heat treatment on the in vitro induced androgenesis in Silene latifolia ssp. alba (en)
- Účinek krátkodobého teplotního působení na indukovanou androgenezi in vitro u Silene latifolia ssp. alba (cs)
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skos:prefLabel
| - The effect of a short heat treatment on the in vitro induced androgenesis in Silene latifolia ssp. alba
- The effect of a short heat treatment on the in vitro induced androgenesis in Silene latifolia ssp. alba (en)
- Účinek krátkodobého teplotního působení na indukovanou androgenezi in vitro u Silene latifolia ssp. alba (cs)
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skos:notation
| - RIV/61389030:_____/05:00024535!RIV06-GA0-61389030
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GV521/96/K117), Z(AV0Z50380511)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/05:00024535
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - campion; green regenerants; ploidy analysis (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Vagera, Jiří
- Kopecký, David
- Šafářová, D.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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is http://linked.open...avai/riv/vysledek
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