About: Localization of male-specifically expressed MROS genes on Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes.

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About: Localization of male-specifically expressed MROS genes on Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The dioecious white campion Silene latifolia (syn. Melandrium album) has heteromorphic sex chromosomes, XX in females and XY in males, that are larger than the autosomes and enable their separation by flow sorting. The group of MROS-genes, the first male-specifically expressed genes in dioecious plants, was recently identified in S. latifolia. To localize the MROSgenes, we used the flow-sorted X chromosomes and autosomes as a template for PCR with internal primers. Our results indicate that the MROS_Y gene is located in at least two copies tandemly arranged on the X chromosome with additional copy(ies) on the autosome(s), while MROS1, MROS2, and MROS4 are exclusively autosomal. The specificity of PCR products was checked by digestion with a restrictionenzyme or reamplification using nested primers. Homology search of databases has shown the presence of five MROS3 homologues in A. thaliana, four of them arranged in two tandems, each consisting of two copies. We conclude that MROS3 is a low-copy gene f The dioecious white campion Silene latifolia (syn. Melandrium album) has heteromorphic sex chromosomes, XX in females and XY in males, that are larger than the autosomes and enable their separation by flow sorting. The group of MROS-genes, the first male-specifically expressed genes in dioecious plants, was recently identified in S. latifolia. To localize the MROSgenes, we used the flow-sorted X chromosomes and autosomes as a template for PCR with internal primers. Our results indicate that the MROS_Y gene is located in at least two copies tandemly arranged on the X chromosome with additional copy(ies) on the autosome(s), while MROS1, MROS2, and MROS4 are exclusively autosomal. The specificity of PCR products was checked by digestion with a restrictionenzyme or reamplification using nested primers. Homology search of databases has shown the presence of five MROS3 homologues in A. thaliana, four of them arranged in two tandems, each consisting of two copies. We conclude that MROS3 is a low-copy gene f (en)
Title	Localization of male-specifically expressed MROS genes on Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes. Localization of male-specifically expressed MROS genes on Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes. (en)
skos:prefLabel	Localization of male-specifically expressed MROS genes on Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes. Localization of male-specifically expressed MROS genes on Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes. (en)
skos:notation	RIV/61989592:15310/01:00001214!RIV/2002/GA0/153102/N
http://linked.open.../vavai/riv/strany	1269-1277
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GV521/96/K117)
http://linked.open...iv/cisloPeriodika	158
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Doležel, Jaroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	685445
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/01:00001214
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	genes (en)
http://linked.open.../riv/klicoveSlovo	genes
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[7438CDF574E8]
http://linked.open...i/riv/nazevZdroje	Genetics resources and crop evolution
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Novel approaches for effective analysis and mapping of genomes in cultivated plants
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	N
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Široký, J. Vrána, J. Souček, R. Matsunaga, S. Kejnovský, E. Vyskot, B.
issn	0925-9864
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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