About: Isolation of chromosomes from Picea abies and their analysis by flow cytometry

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A high-yield method for preparation of suspensions of intact Norway spruce [Picea abies (L.) Karst.] chromosomes was developed for the first time. To accumulate meristem root tip cells at metaphase, actively growing roots were subjected to subsequent treatments with 0.625 mM hydroxyurea for 18 h and after 8 h recovery in distilled water with 0.05 % (m/v) colchicine for 8 h. These treatments resulted in 50 % metaphase indices. Synchronized root tips were fixed in 2 % formaldehyde for 10 min and chromosomes were released into a lysis buffer by mechanical homogenisation, producing 5 x 105 chromosomes from 50 root tips, at average. The isolated chromosomes were morphologically intact and suitable for flow cytometric analysis. Flow karyotypes obtained after the analysis of DAPI-stained chromosomes indicated a possibility to sort at least three different chromosome types. A high-yield method for preparation of suspensions of intact Norway spruce [Picea abies (L.) Karst.] chromosomes was developed for the first time. To accumulate meristem root tip cells at metaphase, actively growing roots were subjected to subsequent treatments with 0.625 mM hydroxyurea for 18 h and after 8 h recovery in distilled water with 0.05 % (m/v) colchicine for 8 h. These treatments resulted in 50 % metaphase indices. Synchronized root tips were fixed in 2 % formaldehyde for 10 min and chromosomes were released into a lysis buffer by mechanical homogenisation, producing 5 x 105 chromosomes from 50 root tips, at average. The isolated chromosomes were morphologically intact and suitable for flow cytometric analysis. Flow karyotypes obtained after the analysis of DAPI-stained chromosomes indicated a possibility to sort at least three different chromosome types. (en) Byla vyvinuta metoda izolace chromosomů z buněk smrku ztepilého (Picea abies). Jako zdroj sloužily kořenové špičky klíčních rosltin. Izolované chromozomy byly morfologicky intaktní a mohly tak být využity pro průtokovou analýzu. Ukázalo se, že pomocí ní je možno izolovat nejmémě tři různé chromozomy smrku ztepilého (cs)
Title	Isolation of chromosomes from Picea abies and their analysis by flow cytometry Isolation of chromosomes from Picea abies and their analysis by flow cytometry (en) Izolace chromozomů z Picea abies a jejich analýza průtokovou cytometrií. (cs)
skos:prefLabel	Isolation of chromosomes from Picea abies and their analysis by flow cytometry Isolation of chromosomes from Picea abies and their analysis by flow cytometry (en) Izolace chromozomů z Picea abies a jejich analýza průtokovou cytometrií. (cs)
skos:notation	RIV/62156489:43210/04:00002614!RIV/2005/GA0/432105/N
http://linked.open.../vavai/riv/strany	199;2003
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GV521/96/K117)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Havel, Ladislav ÜBERALL, Ivo Šmerda, Jakub
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	569075
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/04:00002614
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Norway spruce;chromosome sorting;chromosome suspensions;flow karyotype (en)
http://linked.open.../riv/klicoveSlovo	chromosome suspensions flow karyotype Norway spruce chromosome sorting
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[864BB1AA2D79]
http://linked.open...i/riv/nazevZdroje	Biologia Plantarum
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Novel approaches for effective analysis and mapping of genomes in cultivated plants
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Havel, Ladislav Überall, Ivo Šmerda, Jakub Bartoš, J. Vrána, J.
issn	0006-3134
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	43210

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