About: Preparation of stable Pd nanocubes and their use in biological labeling

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Stable Pd nanocubes (PdNC) with the average size 15 nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres. Stable Pd nanocubes (PdNC) with the average size 15 nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres. (en)
Title	Preparation of stable Pd nanocubes and their use in biological labeling Preparation of stable Pd nanocubes and their use in biological labeling (en)
skos:prefLabel	Preparation of stable Pd nanocubes and their use in biological labeling Preparation of stable Pd nanocubes and their use in biological labeling (en)
skos:notation	RIV/68378050:_____/12:00381927!RIV13-AV0-68378050
http://linked.open...avai/predkladatel	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GA202/09/2078), P(GAP205/10/0348), P(KAN200520704), P(LC545), Z(AV0Z40500505), Z(AV0Z50520514), Z(AV0Z60220518)
http://linked.open...iv/cisloPeriodika	1 December
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Philimonenko, Anatolij Hozák, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	161365
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/12:00381927
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	nanoparticles; palladium nanocubes; electron microscopy (en)
http://linked.open.../riv/klicoveSlovo	electron microscopy nanoparticles palladium nanocubes
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[3CEAAD5EC5B8]
http://linked.open...i/riv/nazevZdroje	Colloids and Surfaces B-Biointerfaces
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Sandwich nanocomposites: preparation, nucleation effects, electrical properties. Functional Organisation of the Cell New nanoparticles for ultrastructural diagnostics Bioinspired nanoparticles sensitive to environment stimuli
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	100
http://linked.open...iv/tvurceVysledku	Hrubý, Martin Hozák, Pavel Nebesářová, Jana Šlouf, Miroslav Philimonenko, Anatoly Bakaeva, Zulfiya Vlková, Helena
http://linked.open...ain/vavai/riv/wos	000307683900029
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni
issn	0927-7765
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.colsurfb.2012.05.023
is http://linked.open...avai/riv/vysledek of	Preparation of stable Pd nanocubes and their use in biological labeling

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