About: Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis

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About: Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Arabinose repressor AraR in Bacillus subtilis negatively controls expression of genes in the metabolic pathway of arabinose containing polysaccharides. The protein is composed of two domains of different phylogenetic origin and function: the N-terminal DNA binding domain belonging to GntR family and the C-terminal effector binding domain, a GalR/LacI family member. We determined the crystal structure of the C-terminal effector-binding domain of AraR in complex with the effector L-arabinose at 2.2 angstrom resolution. The L-arabinose binding affinity was characterized by isothermal titration calorimetry and differential scanning fluorimetry, the Kd value was 8.4 +/- 0.4 microM. Effect of L-arabinose on the protein oligomeric state was investigated in a solution and a detailed analysis of crystal identified dimer organization distinctive from other members of the GalR/LacI family. Arabinose repressor AraR in Bacillus subtilis negatively controls expression of genes in the metabolic pathway of arabinose containing polysaccharides. The protein is composed of two domains of different phylogenetic origin and function: the N-terminal DNA binding domain belonging to GntR family and the C-terminal effector binding domain, a GalR/LacI family member. We determined the crystal structure of the C-terminal effector-binding domain of AraR in complex with the effector L-arabinose at 2.2 angstrom resolution. The L-arabinose binding affinity was characterized by isothermal titration calorimetry and differential scanning fluorimetry, the Kd value was 8.4 +/- 0.4 microM. Effect of L-arabinose on the protein oligomeric state was investigated in a solution and a detailed analysis of crystal identified dimer organization distinctive from other members of the GalR/LacI family. (en)
Title	Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis (en)
skos:prefLabel	Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis (en)
skos:notation	RIV/68378050:_____/12:00376532!RIV13-AV0-68378050
http://linked.open...avai/predkladatel	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ME08016), Z(AV0Z40550506), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Pachl, Petr Fábry, Milan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	171899
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/12:00376532
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	repressor; dimerization; effector binding; isothermal titration calorimetry (en)
http://linked.open.../riv/klicoveSlovo	dimerization repressor effector binding isothermal titration calorimetry
http://linked.open...odStatuVydavatele	DK - Dánské království
http://linked.open...ontrolniKodProRIV	[79AB189B244E]
http://linked.open...i/riv/nazevZdroje	Acta Crystallographica Section D-Biological Crystallography
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Structural studies of transcriptional regulators of the DeoR and GntR families involved in catabolic repression in Bacillus subtilis.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	68
http://linked.open...iv/tvurceVysledku	Fábry, Milan Pachl, Petr Sieglová, Irena Čermáková, Kateřina Řezáčová, Pavlína Otwinowski, Z. Procházková, Kateřina
http://linked.open...ain/vavai/riv/wos	000299469100011
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0907-4449
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1107/S090744491105414X
is http://linked.open...avai/riv/vysledek of	Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis

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