About: Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94     Goto   Sponge   NotDistinct   Permalink

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Description
  • The DbeA1 variant of a novel haloalkane dehalogenase DbeA (EC3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA 110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1,3-dichloropropane.
  • The DbeA1 variant of a novel haloalkane dehalogenase DbeA (EC3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA 110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1,3-dichloropropane. (en)
Title
  • Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94
  • Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94 (en)
skos:prefLabel
  • Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94
  • Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94 (en)
skos:notation
  • RIV/68378050:_____/11:00372330!RIV12-AV0-68378050
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED0001/01/01), P(ED1.1.00/02.0073), P(IAA401630901), P(LC06010), Z(AV0Z50520514), Z(AV0Z60870520), Z(MSM0021622412), Z(MSM0021622413), Z(MSM6007665808)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...dnocenehoVysledku
  • 194016
http://linked.open...ai/riv/idVysledku
  • RIV/68378050:_____/11:00372330
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • crystal-structure; Japonicum; USDA110; enzyme (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [600F15863E83]
http://linked.open...i/riv/nazevZdroje
  • Crystal Growth & Design
http://linked.open...in/vavai/riv/obor
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http://linked.open...vavai/riv/projekt
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http://linked.open...v/svazekPeriodika
  • 11
http://linked.open...iv/tvurceVysledku
  • Chaloupková, R.
  • Damborský, J.
  • Kutá-Smatanová, Ivana
  • Řezáčová, Pavlína
  • Degtjarik, O.
  • Kutý, Michal
  • Nagata, Y.
  • Prudnikova, T.
  • Sato, Y.
http://linked.open...ain/vavai/riv/wos
  • 000286714200021
http://linked.open...n/vavai/riv/zamer
issn
  • 1528-7483
number of pages
http://bibframe.org/vocab/doi
  • 10.1021/cg1013363
is http://linked.open...avai/riv/vysledek of
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