About: Center of Biocatalysis and Biotransformation

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Projekt, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Projekt
rdfs:seeAlso	http://www.isvav.cz/projectDetail.do?rowId=LC06010
Description	The aim of the project is to develop and optimize novel biocatalysts (dehalogenases, esterases, nitrilases, amidases, glycosidases etc.), to study their molecular structure and biocatalysis mechanisms and to introduce methods for their structure-function analysis and goal-directed improvement. Principle methods: screening of new microbial enzymes, enzyme library construction, directed evolution, cloning and expression of genes, protein crystal formation and analysis, molecular modeling, software development and modification, practical applications of enzymes to biotransformations and chemoenzymatic synthesis. The key aspect consists in the education of undergraduate and postgraduate students, the organization of methodical seminars, workshops and scientific symposia and the expansion of international contacts, particularly through participation in international projects. (en) Cílem projektu je vývoj a optimalizace nových biokatalyzátorů (dehalogenas, esteras, nitrilas, amidas, glykosidas a dalších vybraných enzymů), studium mechanismů biokatalýzy a molekulární struktury těchto enzymů, vývoj a zavádění metod pro studium struktury a funkce enzymů a pro jejich cílenou optimalizaci. Hlavní využité metodiky: screening nových mikrobiálních enzymů a vytváření knihoven, metody řízené evoluce, klonování a exprese genů, krystalizace proteinů a následná analýza, molekulární modelování, modifikace a vývoj nového software, chemoenzymová syntéza a biotransformace ověřující praktické aplikace enzymů. Klíčová bude výchova pregraduálních a postgraduálních studentů, organizace metodických seminářů, workshopů a vědeckých kongresů a rozvíjení zahraničních styků především účastí v mezinárodních projektech. V posledním prodlouženém roce řešení (2011)budou dokončeny experimentální práce, zpracována data, finalizovány publikace a doktorské práce.
Title	Center of Biocatalysis and Biotransformation (en) Centrum biokatalýzy a biotransformací
skos:notation	LC06010
http://linked.open...avai/cep/aktivita	Basic Research Centres
http://linked.open...kovaStatniPodpora	http://linked.opendata.cz/resource/domain/vavai/projekt/LC06010/celkovaStatniPodpora
http://linked.open...ep/celkoveNaklady	http://linked.opendata.cz/resource/domain/vavai/projekt/LC06010/celkoveNaklady
http://linked.open...datumDodatniDoRIV	2012-07-03 (xsd:date)
http://linked.open...i/cep/druhSouteze	VS - Veřejná soutěž ve výzkumu a vývoji
http://linked.open...ep/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open.../cep/fazeProjektu	91882673
http://linked.open...ai/cep/hlavniObor	CE - Biochemie
http://linked.open...hodnoceniProjektu	V - Vynikající výsledky (s mezinárodním významem apod.). Zároveň byly splněny cíle a předpokládané výsledky uvedené ve smlouvě / rozhodnutí o poskytnutí podpory.
http://linked.open...vai/cep/kategorie	ZV - Základní výzkum
http://linked.open.../cep/klicovaSlova	enzymová katalýza; katalytická aktivita; proteinové inženýrství; substrátová specifita; exprese genů; řízená evoluce (en)
http://linked.open...ep/partnetrHlavni	Mikrobiologický ústav AV ČR, v.v.i.
http://linked.open...inujicichPrijemcu	1 (xsd:int)
http://linked.open...cep/pocetPrijemcu	4 (xsd:int)
http://linked.open...ocetSpoluPrijemcu	0 (xsd:int)
http://linked.open.../pocetVysledkuRIV	193 (xsd:int)
http://linked.open...enychVysledkuVRIV	193 (xsd:int)
http://linked.open...lneniVMinulemRoce	2011-02-11 (xsd:date)
http://linked.open.../prideleniPodpory	http://linked.opendata.cz/resource/domain/vavai/cep/prideleniPodpory/32341%2F2010-31
http://linked.open...iciPoslednihoRoku	2011
http://linked.open...atUdajeProjZameru	2012
http://linked.open.../vavai/cep/soutez	SMSM200600LC2
http://linked.open...usZobrazovaneFaze	DUU
http://linked.open...ai/cep/typPojektu	P - Projekt výzkumu a vývoje financovaný ze státního rozpočtu
http://linked.open...ep/ukonceniReseni	2011-12-31 (xsd:date)
http://linked.open.../cep/vedlejsiObor	EI - Biotechnologie a bionika BO - Biofyzika
http://linked.open...ep/zahajeniReseni	2006-03-01 (xsd:date)
http://linked.open...jektu+dodavatelem	V rámci 6-ti leté aktivity Centra biokatalýzy a biotransformací bylo vytvořeno přes 150 původních publikací popisujících nové principy biokatalýzy a 4 patenty, dále v Centru pracovalo ca 70 studentů (Bc 20, Mgr 18, PhD 41), kteří obhájili své graduační práce. Centrum pořádalo každoročně odborné kursy a vědecká setkání. Členové centra se zapojili do evropských sítí elitních vědeckých pracovišť. (cs) Center of Biocatalysis and Biotransformation created within 6-y activity over 150 original papers describing new principles of enzymatic catalysis and 4 patents were obtained. Ca 70 students defended their theses within the center activity (Bc 20, Mgr 18, PhD 41). Center organized regular courses and scientific meetings. Center members integrated into European scientific networks of excellence. (en)
http://linked.open...tniCyklusProjektu	ZBBBBKU
http://linked.open.../cep/klicoveSlovo	exprese genů katalytická aktivita proteinové inženýrství substrátová specifita enzymová katalýza
is http://linked.open...vavai/riv/projekt of	Protein Engenneering of Enzymes Protein Engenneering of Enzymes Hydrolysis of benzonitrile herbicides by soil actinobacteria and metabolite toxicity Engineering Enzyme Stability and Resistance to an Organic Cosolvent by Modification of Residues in the Access Tunnel Lincomycin Biosynthesis Involves a Tyrosine Hydroxylating Heme Protein of an Unusual Enzyme Family Fungal nitrilases as biocatalysts: Recent developments Glycosidases: a key to tailored carbohydrates Backbone assignment and secondary structure of the PsbQ protein from Photosystem II Engineering Robust and Efficient Protein Catalysts Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal β-N-acetylhexosaminidase Genome mining for the discovery of new nitrilases in filamentous fungi Glycosidases in Carbohydrate Synthesis: When Organic Chemistry Falls Short Glycosidases in Synthesis of Glycomimetics and Unnatural Carbohydrates Internal structure, hygroscopic and reactive properties of mixed sodium methanesulfonate-sodium chloride particles Molecular mechanisms of the effects of organic solvents on activity of haloalkane dehalogenases Molecular mechanisms of the effects of organic solvents on activity of haloalkane dehalogenases Stereoselectivity and conformational stability of haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA110: the effect of pH and temperature Structural analysis of natural killer cell receptor protein 1 (NKR-P1) extracellular domains suggests a conserved long loop region involved in ligand specificity Purification and characterization of a nitrilase from Aspergillus niger K10 CAVER 2.1 CAVER 2.0 HOTSPOT WIZARD 1.0 Phylogenetic Analysis of Haloalkane Dehalogenases High Occurrence of BRCA1 Intragenic Rearrangements in Hereditary Breast and Ovarian Cancer Syndrome in the Czech Republic Electrochemical Sensing of Chromium-Induced DNA Damage: DNA Strand Breakage by Intermediates of Chromium(VI) Electrochemical Reduction. Exploring the Binding Sites of the Haloalkane Dehalogenase DhlA from Xanthobacter autotrophicus GJ10. Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110. Contribution of the putative inner-pore region to the gating of the Transient Receptor Potential Vanilloid Subtype 1 Channel (TRPV1) Functional changes in the vanilloid receptor subtype 1 channel during and after acute desensitization Structure and dynamics of the N-terminal loop of PsbQ from photosystem II of Spinacia oleracea Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli Copper co-crystallization and divalent metal salts cross-influence effect: A new optimization tool improving crystal morphology and diffraction quality WrbA bridges bacterial flavodoxins and eukaryotic NAD(P)H: quinone oxidoreductases Effect of organic solvents on structure and function of haloalkane dehalogenases Protein Engineering of Haloalkane Dehalogenases: Towards the Change of Reaction Mechanism Thermodynamic Analysis of Enantioselectivity of Haloalkane Dehalogenase DbjA and its Variants DbjA and DbjA+H139A towards Brominated Esters and substituted Bromoalkanes Thermodynamic Analysis of Enantioselectivity of Haloalkane Dehalogenase DbjA and its Variants DbjA and DbjA+H139A towards Brominated Esters and substituted Bromoalkanes Construction of pollutant-degrading bacteria by protein and metabolic engineering Crystallization and structural analysis of DhaA31 from Rhodococcus rhodochrous Crystallization and Preliminary Crystallographic Analysis of Rhodococcus rhodochrous Wild-type DhAa Protein and its Variant DhaA13 Complexed with Different Ligands Computation and Visualization of Surface of Proteins and their Channels Crystallization and crystallographic analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA mutant DhaA31 and its complex with 1,2,3-trichloropropane The C-terminal basic residues contribute to the chemical- and voltage-dependent activation of TRPA1 Computational Modelling of Catalytic Properties and Modified Substrates of Fungal B-N-Acetylhexosaminidases Crystallization and preliminary X-ray diffraction analysis of the wild-type haloalkane dehalogenase DhaA and its variant DhaA13 complexed with different ligands Dendri-RAFTs: a second generation of cyclopeptide-based glycoclusters Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94 Investigation of Ligand Passage in Proteins Investigation of Ligand Passage in Proteins Application of Functional Protein Microarrays for Detection of p53 Mutant Activity Fylogenetická analýza halogenalkandehalogenáz Bioinformatics: Catalyst of Biochemical and Molecular Biologic Research Biomolecular Diversity Keyhole-Lock-Key Model of Enzymatic Catalysis: Engineering of Tunnels in Proteins with Buried Active Sites Second Step of Hydrolytic Dehalogenation in Haloalkane Dehalogenase Investigated by QM/MM Methods Analysis of transactivation capability and conformation of p53 temperature-dependent mutants and their reactivation by amifostine in yeast Comparative Binding Energy (COMBINE) Analysis of the Substrate Specificity of Haloalkane Dehalogenase from Bradyrhizobium japonicum USDA110. Crystals of DhaA Mutants from Rhodococcus rhodochrous NCIMB 13064 Diffracted to Ultrahigh Resolution: Crystallization and Preliminary Diffraction Analysis.

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