About: Genome mining for the discovery of new nitrilases in filamentous fungi

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Genome mining for the discovery of new nitrilases in filamentous fungi Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Our aim is to describe new fungal nitrilases whose sequences were published but whose catalytic properties were unknown. We adapted for expression in E. coli three of the genes and confirmed that the enzymes acted on organic nitriles. The genome mining approach was used to search for nitrilases in filamentous fungi. Synthetic genes encoding nitrilases in Aspergillus niger, Gibberella moniliformis and Neurospora crassa were expressed in Escherichia coli. This is the first heterologous expression of fungal enzymes of this type. The recombinant enzyme derived from G. moniliformis was an aromatic nitrilase with an aktivity of 390 U l culture with benzonitrile as substrate. This was much less than the activities of the recombinant enzymes derived from A. niger and N. crassa that had activities of 2500 and 2700 U l culture, respectively, with phenylacetonitrile as substrate Our aim is to describe new fungal nitrilases whose sequences were published but whose catalytic properties were unknown. We adapted for expression in E. coli three of the genes and confirmed that the enzymes acted on organic nitriles. The genome mining approach was used to search for nitrilases in filamentous fungi. Synthetic genes encoding nitrilases in Aspergillus niger, Gibberella moniliformis and Neurospora crassa were expressed in Escherichia coli. This is the first heterologous expression of fungal enzymes of this type. The recombinant enzyme derived from G. moniliformis was an aromatic nitrilase with an aktivity of 390 U l culture with benzonitrile as substrate. This was much less than the activities of the recombinant enzymes derived from A. niger and N. crassa that had activities of 2500 and 2700 U l culture, respectively, with phenylacetonitrile as substrate (en)
Title	Genome mining for the discovery of new nitrilases in filamentous fungi Genome mining for the discovery of new nitrilases in filamentous fungi (en)
skos:prefLabel	Genome mining for the discovery of new nitrilases in filamentous fungi Genome mining for the discovery of new nitrilases in filamentous fungi (en)
skos:notation	RIV/61388971:_____/11:00360696!RIV12-AV0-61388971
http://linked.open...avai/predkladatel	Mikrobiologický ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(FT-TA5/043), P(GD305/09/H008), P(IAA500200708), P(LC06010), P(OC09046), S, Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Veselá, Alícia Barbara Kaplan, Ondřej Martínková, Ludmila Bezouška, Karel Felsberg, Jürgen Křen, Vladimír Rinágelová, Anna Petříčková, Alena Malandra, Anna
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	201036
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/11:00360696
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Aromatic nitrilase; Arylacetonitrilase; Aspergillus niger (en)
http://linked.open.../riv/klicoveSlovo	Aromatic nitrilase Arylacetonitrilase Aspergillus niger
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[EF738EDF7AA2]
http://linked.open...i/riv/nazevZdroje	Biotechnology Letters
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	9 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Research of innovative techniques for elimination of benzonitrile pesticides from the environment. Nitrilases from filamentous fungi: cloning, expression, characterization and directed evolution Preparation, biotransformation, and optimalization of compounds with antitumor and antimicrobial effects Center of Biocatalysis and Biotransformation Cascade chemoenzymatic reactions of nitriles
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	33
http://linked.open...iv/tvurceVysledku	Bezouška, Karel Křen, Vladimír Kaplan, Ondřej Martínková, Ludmila Petříčková, Alena Veselá, Alícia Barbara Felsberg, Jürgen Malandra, Anna Rinágelová, Anna
http://linked.open...ain/vavai/riv/wos	000286198700014
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	0141-5492
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10529-010-0421-7
is http://linked.open...avai/riv/vysledek of	Genome mining for the discovery of new nitrilases in filamentous fungi Genome mining for the discovery of new nitrilases in filamentous fungi

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software