About: Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli

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Description	EcoR124I is a multicomplex enzyme belonging to the type I restriction-modification system from Escherichia coli. Although EcoR124I has been extensively characterized biochemically, there is no direct structural information available about particular subunits. HsdR is a motor subunit that is responsible for ATP hydrolysis, DNA translocation and cleavage of the DNA substrate recognized by the complex. Recombinant HsdR subunit was crystallized using the sitting-drop vapour-diffusion method. Crystals belong to the primitive monoclinic space group, with unit-cell parameters a = 85.75, b = 124.71, c = 128.37 angstrom, beta = 108.14 degrees. Native data were collected to 2.6 angstrom resolution at the X12 beamline of EMBL Hamburg. EcoR124I is a multicomplex enzyme belonging to the type I restriction-modification system from Escherichia coli. Although EcoR124I has been extensively characterized biochemically, there is no direct structural information available about particular subunits. HsdR is a motor subunit that is responsible for ATP hydrolysis, DNA translocation and cleavage of the DNA substrate recognized by the complex. Recombinant HsdR subunit was crystallized using the sitting-drop vapour-diffusion method. Crystals belong to the primitive monoclinic space group, with unit-cell parameters a = 85.75, b = 124.71, c = 128.37 angstrom, beta = 108.14 degrees. Native data were collected to 2.6 angstrom resolution at the X12 beamline of EMBL Hamburg. (en) EcoR124I je multikomplexní enzym patřící k Typu 1 restrikčně-modifikačních systémů z E.coli. Ačkoliv je EcoR124I charakterizován velmi dobře z biochemického hlediska, neexistují žádné přímé informace o jednotlivých podjednotkách. HsdR je hnací podjednotka, která je odpovědná za hydrolýzu ATP, translokaci DNA a štěpení DNA substrátu rozpoznaného komplexem. HsdR podjednotka byla krystalizována použitím metody sedící kapky založené na difuzy par. Krystaly tvoří primitivní monoklinickou prostorovou grupu s mřížkovými parametry a = 85.75 Ǻ, b = 124.71 Ǻ, c = 128.37 Ǻ, β = 108.14°. Nativní data set byl naměřen do rozlišení 2.6 Ǻ na beamline X12 synchrotronu EMBL/DESY v Hamburku (cs)
Title	Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli Purifikace, krystalizace a X-ray analýza HsdR podjednotky EcoR124I endonucleázy z E. coli (cs) Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli (en)
skos:prefLabel	Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli Purifikace, krystalizace a X-ray analýza HsdR podjednotky EcoR124I endonucleázy z E. coli (cs) Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli (en)
skos:notation	RIV/67179843:_____/07:00098095!RIV08-AV0-67179843
http://linked.open.../vavai/riv/strany	582;585
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(LC06010), S, Z(AV0Z60870520), Z(MSM6007665808)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Lapkouski, Mikalai
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Centrum výzkumu globální změny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	446025
http://linked.open...ai/riv/idVysledku	RIV/67179843:_____/07:00098095
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA translocation (en)
http://linked.open.../riv/klicoveSlovo	DNA translocation
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[9BA234A26C29]
http://linked.open...i/riv/nazevZdroje	Acta Crystallographica Section F
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of Biocatalysis and Biotransformation
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	63
http://linked.open...iv/tvurceVysledku	Lapkouski, Mikalai
http://linked.open...n/vavai/riv/zamer	Spatial and functional dynamics of biological, ecological and socio-economic systems interacting with global climatic change Fyzikální biologie -nové přístupy v biologickém výzkumu
issn	1744-3091
number of pages	4 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli

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