About: Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus

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About: Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	IRS-2 from the hard tick Ixodes ricinus belongs into the serpin family of protease inhibitors. It is produced in the tick salivary glands, and its anti-inflammatory activity suggests a role in the parasitehost interaction. Recombinant IRS-2 prepared by heterologous expression in bacterial system was crystallized and crystals diffracted to resolution 1.8 Å. IRS-2 was cleaved during crystallization by contaminating proteases. This processing of IRS-2 mimicked the specific cleavage of serpin by its target protease and resulted in a more stable R conformation, which produced well-diffracting crystals. Activity profiling with specific substrates and inhibitors demonstrated traces of serine and cysteine proteases in the protein stock solution. IRS-2 from the hard tick Ixodes ricinus belongs into the serpin family of protease inhibitors. It is produced in the tick salivary glands, and its anti-inflammatory activity suggests a role in the parasitehost interaction. Recombinant IRS-2 prepared by heterologous expression in bacterial system was crystallized and crystals diffracted to resolution 1.8 Å. IRS-2 was cleaved during crystallization by contaminating proteases. This processing of IRS-2 mimicked the specific cleavage of serpin by its target protease and resulted in a more stable R conformation, which produced well-diffracting crystals. Activity profiling with specific substrates and inhibitors demonstrated traces of serine and cysteine proteases in the protein stock solution. (en)
Title	Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus (en)
skos:prefLabel	Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus (en)
skos:notation	RIV/68378050:_____/10:00349395!RIV11-GA0-68378050
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GAP207/10/2183), P(LC06009), Z(AV0Z40550506), Z(AV0Z50520514), Z(AV0Z60220518)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Brynda, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	252450
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/10:00349395
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	protease inhibitor; serpin; tick; proteolysis (en)
http://linked.open.../riv/klicoveSlovo	tick proteolysis serpin protease inhibitor
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[98B5AD9DD012]
http://linked.open...i/riv/nazevZdroje	Acta Crystallographica Section F
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Structural proteomics of proteolytic systems in ticks Centre for molecular ecology of vectors and pathogens
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	F66
http://linked.open...iv/tvurceVysledku	Brynda, Jiří Chmelař, Jindřich Kovářová, Zuzana Mareš, Michael Řezáčová, Pavlína Šanda, Miloslav
http://linked.open...ain/vavai/riv/wos	000283714100011
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni
issn	1744-3091
number of pages	5 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus

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