About: Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples

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About: Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We present a highly sensitive electrochemical genosensor for direct measurement of specific DNA sequences in undiluted and untreated human serum and urine samples. The method relies on a new ternary interface involving hexanedithiol co-immobilized with the thiolated capture probe on gold surfaces, followed by the incorporation of 6-mercapto-1-hexanol as diluent. The new ternary layer led to a 80-fold improvement in the signal-to-noise ratio for 1 nM target DNA in undiluted human serum over the common binary alkanethiol interface, and allowed the direct quantification of the target DNA down to 7 pM (28 amol) and 17pM (68 amol) in untreated serum and urine, respectively. We present a highly sensitive electrochemical genosensor for direct measurement of specific DNA sequences in undiluted and untreated human serum and urine samples. The method relies on a new ternary interface involving hexanedithiol co-immobilized with the thiolated capture probe on gold surfaces, followed by the incorporation of 6-mercapto-1-hexanol as diluent. The new ternary layer led to a 80-fold improvement in the signal-to-noise ratio for 1 nM target DNA in undiluted human serum over the common binary alkanethiol interface, and allowed the direct quantification of the target DNA down to 7 pM (28 amol) and 17pM (68 amol) in untreated serum and urine, respectively. (en)
Title	Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples (en)
skos:prefLabel	Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples (en)
skos:notation	RIV/68081707:_____/11:00373461!RIV12-AV0-68081707
http://linked.open...avai/predkladatel	Biofyzikální ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ME09038), Z(AV0Z50040507), Z(AV0Z50040702)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Paleček, Emil Bartošík, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	234831
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/11:00373461
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	electrochemical detection; DNA hybridization; self-assembled monolayer (en)
http://linked.open.../riv/klicoveSlovo	electrochemical detection self-assembled monolayer DNA hybridization
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[29BA57D98C4F]
http://linked.open...i/riv/nazevZdroje	Biosensors and Bioeletronics
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Interactions of proteins and nucleic acids with surfaces. New tools for biomedicine
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	26
http://linked.open...iv/tvurceVysledku	Wang, J. Paleček, Emil Bartošík, Martin Campuzano, S. Haake, D. A. Kuralay, F. Lobo-Castanón, M. J. Vyavahare, K.
http://linked.open...ain/vavai/riv/wos	000289863900030
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems Genome and epigenome: 1D and 3D Structure, Dynamics, Interactions with Proteins and Functions
issn	0956-5663
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.bios.2011.02.004
is http://linked.open...avai/riv/vysledek of	Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples

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