About: Purification and characterization of a nitrilase from Fusarium solani O1

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	An intracellular nitrilase was purified from a Fusarium solani O1 culture, in which the enzyme (up to 3000 U/L) was induced by 2-cyanopyridine. SDS-PAGE revealed one major band corresponding to a molecular weight of approximately 40 kDa. Peptide mass fingerprinting suggested a high similarity of the protein with the putative nitrilase from Gibberella moniliformis. Electron microscopy revealed that the enzyme molecules associated into extended rods. The enzyme showed the highest specific activities towards benzonitrile (156 U mg-1) and 4-cyanopyridine (203 U mg-1) An intracellular nitrilase was purified from a Fusarium solani O1 culture, in which the enzyme (up to 3000 U/L) was induced by 2-cyanopyridine. SDS-PAGE revealed one major band corresponding to a molecular weight of approximately 40 kDa. Peptide mass fingerprinting suggested a high similarity of the protein with the putative nitrilase from Gibberella moniliformis. Electron microscopy revealed that the enzyme molecules associated into extended rods. The enzyme showed the highest specific activities towards benzonitrile (156 U mg-1) and 4-cyanopyridine (203 U mg-1) (en) Intracelulární nitrilasy byla purifikována z kultury Fusaria solani O1, kde byl enzym indukován 2-kyanopyridinem (až 3000 U/l). Pomocí SDS-PAGE byl získán hlavní proužek o molekulové hmotnosti blížící se 40 kDa. Hmotové spektrum proteinů vykazovalo vysokou podobnost s proteinovou sekvencí nitrrilasy Gibberella moniliformis. Pomocí elektronové mikroskopie bylo zjištěno tvoření agregátů tohoto enzymu v podobě podlouhlých tyček. Enzym vykazoval nejvyšší aktivitu pro benzonitril (156 U/mg) a 4-kyanopyridin (203 U/mg) (cs)
Title	Purification and characterization of a nitrilase from Fusarium solani O1 Vyčištění a popis nitrilasy z houby Fusarium solani O1 (cs) Purification and characterization of a nitrilase from Fusarium solani O1 (en)
skos:prefLabel	Purification and characterization of a nitrilase from Fusarium solani O1 Vyčištění a popis nitrilasy z houby Fusarium solani O1 (cs) Purification and characterization of a nitrilase from Fusarium solani O1 (en)
skos:notation	RIV/61388971:_____/08:00313082!RIV09-AV0-61388971
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0505), P(GA203/05/2267), P(IAA500200708), P(LC06010), P(OC D25.002), Z(AV0Z50200510), Z(MSM0021620808)
http://linked.open...iv/cisloPeriodika	2-4
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Uhnáková, Bronislava Kaplan, Ondřej Martínková, Ludmila Vejvoda, Vojtěch Bezouška, Karel Křen, Vladimír Šulc, Miroslav Rinágelová, Anna Pompach, Petr Benada, Oldřich
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	391077
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/08:00313082
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	nitrilase; fusarium solani; nitrile hydrolysis (en)
http://linked.open.../riv/klicoveSlovo	nitrile hydrolysis fusarium solani nitrilase
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[5067FE5992F8]
http://linked.open...i/riv/nazevZdroje	Journal of Molecular Catalysis B-Enzymatic
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	10 (xsd:int)
http://linked.open...cetTvurcuVysledku	13 (xsd:int)
http://linked.open...vavai/riv/projekt	Nitrilases from filamentous fungi: cloning, expression, characterization and directed evolution Center of targeted therapeutic drugs Center of Biocatalysis and Biotransformation http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20D25.002 Enzymes catalyzing stereoselective transformations of C-N bonds in organic compounds and the use of these biocatalysts to prepare chiral synthons
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	50
http://linked.open...iv/tvurceVysledku	Benada, Oldřich Bezouška, Karel Fischer, L. Křen, Vladimír Kaplan, Ondřej Lutz-Wahl, S. Martínková, Ludmila Uhnáková, Bronislava Vejvoda, Vojtěch Šulc, Miroslav Pompach, Petr Rinágelová, Anna Cantarella, M.
http://linked.open...ain/vavai/riv/wos	000252487300009
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Molekulárně biologické, genetické a epigenetické aspekty vzniku a rozvoje modelových tumorů dospělého věku. Význam pro epidemiologii, časnou diagnostiku a léčbu
issn	1381-1177
number of pages	8 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Purification and characterization of a nitrilase from Fusarium solani O1 Purification and characterization of a nitrilase from Fusarium solani O1 Purification and characterization of a nitrilase from Fusarium solani O1 Purification and characterization of a nitrilase from Fusarium solani O1

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