About: Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	IscA/Isa proteins function as alternative scaffolds for the assembly of Fe-S clusters and/or provide iron for their assembly in prokaryotes and eukaryotes. We have studied their function in Trypanosoma brucei, where the requirement for both of them to sustain cell growth depends on the life cycle stage. Reactive oxygen species but not iron accumulate in the procyclic mitochondrion upon ablation of the TbIsa proteins, but their depletion does not influence the assembly of Fe-S clusters in cytosolic proteins. In the bloodstream form, which has a downregulated mitochondrion, the TbIsa proteins are non-essential. The Isa2 orthologue of the anaerobic protist Blastocystis partially rescued the growth and enzymatic activities of TbIsa1/2 knock-down. Rescues with human Isa orthologues partially recovered the activities of aconitase and fumarase. These results show that the Isa1 and Isa2 proteins of diverse eukaryotes have overlapping functions. IscA/Isa proteins function as alternative scaffolds for the assembly of Fe-S clusters and/or provide iron for their assembly in prokaryotes and eukaryotes. We have studied their function in Trypanosoma brucei, where the requirement for both of them to sustain cell growth depends on the life cycle stage. Reactive oxygen species but not iron accumulate in the procyclic mitochondrion upon ablation of the TbIsa proteins, but their depletion does not influence the assembly of Fe-S clusters in cytosolic proteins. In the bloodstream form, which has a downregulated mitochondrion, the TbIsa proteins are non-essential. The Isa2 orthologue of the anaerobic protist Blastocystis partially rescued the growth and enzymatic activities of TbIsa1/2 knock-down. Rescues with human Isa orthologues partially recovered the activities of aconitase and fumarase. These results show that the Isa1 and Isa2 proteins of diverse eukaryotes have overlapping functions. (en)
Title	Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues (en)
skos:prefLabel	Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues (en)
skos:notation	RIV/60077344:_____/11:00365995!RIV12-MSM-60077344
http://linked.open...avai/predkladatel	Biologické centrum AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(2B06129), P(GA204/09/1667), P(LC07032), S, Z(AV0Z60220518), Z(MSM6007665801)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Lukeš, Julius Verner, Zdeněk Long, Shaojun Skalický, Tomáš Changmai, Piya Wen, Yan-Zi
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	231906
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/11:00365995
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	IRON-SULFUR CLUSTER; ESCHERICHIA-COLI; ASSEMBLY PROTEIN; SACCHAROMYCES-CEREVISIAE; AZOTOBACTER-VINELANDII; CYSTEINE DESULFURASE; CRYSTAL-STRUCTURE; BINDING ACTIVITY; GENE-CLUSTER (en)
http://linked.open.../riv/klicoveSlovo	CRYSTAL-STRUCTURE SACCHAROMYCES-CEREVISIAE ASSEMBLY PROTEIN AZOTOBACTER-VINELANDII BINDING ACTIVITY CYSTEINE DESULFURASE GENE-CLUSTER IRON-SULFUR CLUSTER ESCHERICHIA-COLI
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[53257E304977]
http://linked.open...i/riv/nazevZdroje	Molecular Microbiology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Model organisms as a key to the understanding of functions of disease-related human genes Characterization of a novel protein complex involved in RNA processing and editing in the mitochondrion of Trypanosoma brucei Center for Functional Genetics
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	81
http://linked.open...iv/tvurceVysledku	Lukeš, Julius Verner, Zdeněk Skalický, Tomáš Changmai, Piya Long, Shaojun Roger, A. J. Tsaousis, A. D. Wen, Yan-Zi
http://linked.open...ain/vavai/riv/wos	000295087000001
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni EKOLOGICKÉ, EVOLUČNÍ A EXPERIMENTÁLNĚ BIOLOGICKÉ PŘÍSTUPY KE STUDIU VZNIKU A VÝZNAMU BIODIVERZITY
issn	0950-382X
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/j.1365-2958.2011.07769.x
is http://linked.open...avai/riv/vysledek of	Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software