About: Burkholderia cenocepacia lectin D     Goto   Sponge   NotDistinct   Permalink

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Description
  • Gene for Burkholderia cenocepacia lectin D (BclD) was cloned in pRSET vector and expressed. However, expression was difficult, with very low yield and protein was produced mainly in insoluble form. BclD contains N-terminal domain of unknown function. As expression of lectins domains are generally high, we decided to separate N-terminal and lectin domain. Several crystals of lectin domain in different conditions were obtained using hanging drop vapour diffusion technique. These crystals are ready for data collection at ESRF (European Synchrotron Radiation Facility).
  • Gene for Burkholderia cenocepacia lectin D (BclD) was cloned in pRSET vector and expressed. However, expression was difficult, with very low yield and protein was produced mainly in insoluble form. BclD contains N-terminal domain of unknown function. As expression of lectins domains are generally high, we decided to separate N-terminal and lectin domain. Several crystals of lectin domain in different conditions were obtained using hanging drop vapour diffusion technique. These crystals are ready for data collection at ESRF (European Synchrotron Radiation Facility). (en)
Title
  • Burkholderia cenocepacia lectin D
  • Burkholderia cenocepacia lectin D (en)
skos:prefLabel
  • Burkholderia cenocepacia lectin D
  • Burkholderia cenocepacia lectin D (en)
skos:notation
  • RIV/00216224:14310/08:00024966!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA303/06/0570), P(ME08008), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika
  • 3a
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 358650
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/08:00024966
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Burkholderia cenocepacia; lectin; structure (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [E2EC73703E9A]
http://linked.open...i/riv/nazevZdroje
  • Materials Structure in Chemistry, Biology, Physics and Technology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 15
http://linked.open...iv/tvurceVysledku
  • Malinovská, Lenka
  • Pokorná, Marie
  • Wimmerová, Michaela
http://linked.open...n/vavai/riv/zamer
issn
  • 1211-5894
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
is http://linked.open...avai/riv/vysledek of
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