| Attributes | Values |
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| rdf:type
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| rdfs:seeAlso
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| Description
| - Nitrilases from Aspergillus niger CBS 513.88, A. niger K10, Gibberella moniliformis, Neurospora crassa OR74A, and Penicillium marneffei ATCC 18224 were expressed in Escherichia coli BL21-Gold (DE3) after IPTG induction. N. crassa nitrilase exhibited the highest yield of 69,000 ULMINUS SIGN 1 culture. Co-expression of chaperones (GroEL/ES in G. moniliformis and P. marneffei; GroEL/ ES and trigger factor in N. crassa and A. niger CBS 513.88) enhanced the enzyme solubility. Specific activities of strains expressing the former two enzymes increased approximately fourfold upon co-expression of GroEL/ES. The enzyme from G. moniliformis (co-purified with GroEL) preferred benzonitrile as substrate (Km of 0.41 mM, Vmax of 9.7 μmol minMINUS SIGN 1 mgMINUS SIGN 1 protein). The P. marneffei enzyme (unstable in its purified state) exhibited the highest Vmax of 7.3 μmol minMINUS SIGN 1 mgMINUS SIGN 1 protein in cellfree extract, but also a high Km of 15.4 mM, for 4- cyanopyridine. The purified nitrilases from A. niger CBS 513.88 and N. crassa acted preferentially on phenylacetonitrile (Km of 3.4 and 2.0 mM, respectively; Vmax of 10.6 and 17.5 μmol minMINUS SIGN 1 mgMINUS SIGN 1 protein, respectively), and hydrolyzed also (R,S)-mandelonitrile with higher Km values. Significant amounts of amides were only formed by the G. Moniliformis nitrilase from phenylacetonitrile and 4-cyanopyridine.
- Nitrilases from Aspergillus niger CBS 513.88, A. niger K10, Gibberella moniliformis, Neurospora crassa OR74A, and Penicillium marneffei ATCC 18224 were expressed in Escherichia coli BL21-Gold (DE3) after IPTG induction. N. crassa nitrilase exhibited the highest yield of 69,000 ULMINUS SIGN 1 culture. Co-expression of chaperones (GroEL/ES in G. moniliformis and P. marneffei; GroEL/ ES and trigger factor in N. crassa and A. niger CBS 513.88) enhanced the enzyme solubility. Specific activities of strains expressing the former two enzymes increased approximately fourfold upon co-expression of GroEL/ES. The enzyme from G. moniliformis (co-purified with GroEL) preferred benzonitrile as substrate (Km of 0.41 mM, Vmax of 9.7 μmol minMINUS SIGN 1 mgMINUS SIGN 1 protein). The P. marneffei enzyme (unstable in its purified state) exhibited the highest Vmax of 7.3 μmol minMINUS SIGN 1 mgMINUS SIGN 1 protein in cellfree extract, but also a high Km of 15.4 mM, for 4- cyanopyridine. The purified nitrilases from A. niger CBS 513.88 and N. crassa acted preferentially on phenylacetonitrile (Km of 3.4 and 2.0 mM, respectively; Vmax of 10.6 and 17.5 μmol minMINUS SIGN 1 mgMINUS SIGN 1 protein, respectively), and hydrolyzed also (R,S)-mandelonitrile with higher Km values. Significant amounts of amides were only formed by the G. Moniliformis nitrilase from phenylacetonitrile and 4-cyanopyridine. (en)
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| Title
| - Purification and characterization of heterologously expressed nitrilases from filamentous fungi
- Purification and characterization of heterologously expressed nitrilases from filamentous fungi (en)
|
| skos:prefLabel
| - Purification and characterization of heterologously expressed nitrilases from filamentous fungi
- Purification and characterization of heterologously expressed nitrilases from filamentous fungi (en)
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| skos:notation
| - RIV/00216208:11310/12:10104142!RIV13-GA0-11310___
|
| http://linked.open...avai/predkladatel
| |
| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - I, P(GAP504/11/0394), P(GD305/09/H008), P(IAA500200708), P(LC06010), P(OC09046), S, Z(AV0Z50200510)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11310/12:10104142
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Penicillium marneffei; Neurospora crassa; Gibberella moniliformis; Aspergillus niger; Chaperones; Nitrilase (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Applied Microbiology and Biotechnology
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Bezouška, Karel
- Křen, Vladimír
- Kaplan, Ondřej
- Martínková, Ludmila
- Petříčková, Alena
- Uhnáková, Bronislava
- Felsberg, Jürgen
- Malandra, Anna
- Rinágelová, Anna
- Veselá, Alicja Barbara
- Kubáč, David
- Weyrauch, Philip
|
| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| http://bibframe.org/vocab/doi
| - 10.1007/s00253-011-3525-7
|
| http://localhost/t...ganizacniJednotka
| |
| is http://linked.open...avai/riv/vysledek
of | |
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