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Description
  • In this chapter, the basic principles and protocols of the electron microscopical detections of specific DNA and RNA sequences are described. We focused primarily on a comparison of various methods of electron microscopy in situ hybridization (EM-ISH) with respect to their sensitivity and the structural preservation of the sample with the aim of helping the readers select the appropriate hybridization protocol. As the post-embedding EM-ISH most frequently represents the optimal choice, the protocol for the post-embedding EM-ISH approach is described in detail. Concurrently, the alternative methods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail.
  • In this chapter, the basic principles and protocols of the electron microscopical detections of specific DNA and RNA sequences are described. We focused primarily on a comparison of various methods of electron microscopy in situ hybridization (EM-ISH) with respect to their sensitivity and the structural preservation of the sample with the aim of helping the readers select the appropriate hybridization protocol. As the post-embedding EM-ISH most frequently represents the optimal choice, the protocol for the post-embedding EM-ISH approach is described in detail. Concurrently, the alternative methods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail. (en)
Title
  • Tracking DNA and RNA Sequences at High Resolution
  • Tracking DNA and RNA Sequences at High Resolution (en)
skos:prefLabel
  • Tracking DNA and RNA Sequences at High Resolution
  • Tracking DNA and RNA Sequences at High Resolution (en)
skos:notation
  • RIV/00216208:11110/14:10284235!RIV15-MSM-11110___
http://linked.open...avai/riv/aktivita
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  • I, P(ED0030/01/01), P(ED1.1.00/02.0109), P(GAP302/12/1885), P(GBP302/12/G157), P(TA03010598), P(TA03010719)
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  • 50894
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  • RIV/00216208:11110/14:10284235
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  • Freeze substitution; High-pressure freezing; Cryo-sections; Pre-embedding; Post-embedding; In situ hybridization; Double- and single-stranded DNA and RNA; Electron microscopy (en)
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  • [85E191CA1F00]
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  • New York
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  • Methods in Molecular Biology, 1117
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  • Electron Microscopy; Methods and Protocols : Methods and Protocols
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  • Cmarko, Dušan
  • Koberna, Karel
  • Ligasová, Anna
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/978-1-62703-776-1_16
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  • Humana Press
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  • 978-1-62703-775-4
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  • 11110
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