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| Description
| - The main aim of the proposed project is to optimize the unconventional methods of hybridization via protoplast fusion techniques in economically important cultivated crops of the genus Brassica, Cucumis and Solanum. The methods of protoplast technology should help in developing the specific gene combinations in studied plant species, which are impossible to obtain through the conventional breeding approaches. Further this techniques offer a convenient way for overcoming the limitations on sexual hybridization in plant breeding. The goal of protoplast fusion is to control the transfer of an agriculturally important traits, such as disease resistance, abiotic stresses and also the improvement of the quality characteristics of seeds. The proposed investigations of this research are focussed on the development of the methods for symetrical and asymetrical protoplast fusion. The fusions are supposed to be induced through both electroporation (electrical stimulation) as well as chemical polyetylenglycol(PEG (en)
- Cílem projektu je optimalizovat netradiční metody hybridizace pomocí fúze protoplastů u hospodářsky významných plodin. Metoda má přispět ke vzniku jedinečných kombinací genů různých rostlinných druhů, které není možné získat cestou klasického křížení a nabízí překlenutí sexuálních bariér ve šlechtění rostlin. Cílem fúzí protoplastů je přenos významných znaků rezistencí vůči patogenům, abiotickým stresům a kvalita semene. Cílem tohoto výzkumu je vyvinout metodiky pro symetrickou a asymetrickou fúzi protoplastů jak elektrickým polem, tak chemicky u zemědělských plodin a planých druhů rodu Brassica, Cucumis a Solanum. Vybrané genotypy jednotlivých plodin budou hodnoceny z hlediska výtěžnosti, životnosti a regenerace protoplastů. Průběh somatické hybridizace bude sledován cytologicky. Hybridnost produktů fúzí a případně získaných regenerantů bude ověřovány cytologicky a také metodami molekulární biologie. Vytvořené rostlinné materiály mohou přispět k rozšíření genetické variability u použitých hospodá
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| Title
| - Utilization of protoplast fusion technique in breeding of the important cultivated crops of the genera Brassica, Cucumis and Solanum. (en)
- Využití techniky fúze protoplastů ve šlechtění hospodářsky významných plodin rodu Brassica, Cucumis a Solanum
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| skos:notation
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| http://linked.open...avai/cep/aktivita
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| http://linked.open...kovaStatniPodpora
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| http://linked.open...ep/celkoveNaklady
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| http://linked.open...datumDodatniDoRIV
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| http://linked.open...i/cep/druhSouteze
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| http://linked.open...ep/duvernostUdaju
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| http://linked.open.../cep/fazeProjektu
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| http://linked.open...ai/cep/hlavniObor
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| http://linked.open...hodnoceniProjektu
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| http://linked.open...vai/cep/kategorie
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| http://linked.open.../cep/klicovaSlova
| - Brassica; Cucumis; Solanum; izolace protoplastů; fúze protoplastů; somatická hybridizace; cybridizace; ploidie; haploid; rezistence k chorobám a abiotickým faktorům (en)
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| http://linked.open...ep/partnetrHlavni
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| http://linked.open...inujicichPrijemcu
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| http://linked.open...cep/pocetPrijemcu
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| http://linked.open...ocetSpoluPrijemcu
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| http://linked.open.../pocetVysledkuRIV
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| http://linked.open...enychVysledkuVRIV
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| http://linked.open...lneniVMinulemRoce
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| http://linked.open.../prideleniPodpory
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| http://linked.open...iciPoslednihoRoku
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| http://linked.open...atUdajeProjZameru
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| http://linked.open.../vavai/cep/soutez
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| http://linked.open...usZobrazovaneFaze
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| http://linked.open...ai/cep/typPojektu
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| http://linked.open...ep/ukonceniReseni
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| http://linked.open.../cep/vedlejsiObor
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| http://linked.open...ep/zahajeniReseni
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| http://linked.open...jektu+dodavatelem
| - Řešení probíhalo v souladu s plánem projektu. V rámci rodů Brassica, Cucumis a Solanum byla fúzována řada kombinací, vedoucí u rodů Brassica a Solanum k regeneraci rostlin, u rodu Cucumis do stádia kalusů. Byla dosažena řada originálních výsledků. (cs)
- Solution proceeded acording to plan of project. Within family Brassica, Cucumis and Solanum were fused a lot of combination, which faces by family Brassica and Solanum to regeneration of plants, by family Cucumis to phase od caluss. Original outcomes wer (en)
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| http://linked.open...tniCyklusProjektu
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| http://linked.open.../cep/klicoveSlovo
| - Brassica
- Solanum
- Cucumis
- cybridizace
- fúze protoplastů
- haploid
- izolace protoplastů
- ploidie
- somatická hybridizace
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| is http://linked.open...vavai/riv/projekt
of | - Gajdová J., Lebeda A., Navrátilová B. 2004: Protoplast cultures of Cucumis and Cucurbita spp. In: Lebeda, A., Paris, H.S. (Eds.): Progress in Cucurbit Genetics and Breeding Research. Proceedings of Cucurbitaceae 2004, the 8th EUCARPIA Meeting on Cucurbi
- Gajdová J., Navrátilová B., Lebeda A. 2004: Progress in research of Cucurbitaceae protoplast cultures. Acta Physiol. Plantarum 26 (No. 3 Supplement), (Abstract; The 14th FESPB Congress, Book of Abstracts, August 23-27, 2004 Cracow, Poland), p. 250.
- Gajdová J., Navrátilová B., Smolná J. 2004. Callus culture and protoplast isolation of Cucumis species. Book of abstracts. Xth days of plant physiology. Bratislava 5-9.9. 2004, Bratislava, SR, 22.
- Presence of a translocation from Aegilops ventricosa in wheat cultivars registered in the Czech Republic
- Luhová L., Petřivalský M., Navrátilová B. 2005. Localisation of NO production and NOS activity in cucumber protoplast. Journal of Applied Biomedicine, Vol. 3, Suppl. 1 (Cell VI), pp. 30-31.
- Navrátilová B., Greplová M., Klíma M., Vyvadilová M., Gajdová J., Skálová D. 2005. Contribution of somatic hybridization to improvement of selected cultural crops. In: From laboratory to business, Book of Abstracts, 6th Int Symp in the Series Recent adv
- ACCLIMATIZATION OF SOMATIC HYBRIDS AND PLANTLETS AFTER CHROMOSOME DOUBLING IN SOLANUM GENUS
- Interspecific hybridization of C. anguria x C. zeyheri, C. sativus x C. melo and C. sativus x C. metuliferus with the use of embryo cultures
- Metody izolace pylových zrn Cucumis spp. (C. sativus a C. melo) využitelné při in vitro opylování
- Effect of genotype, source tissue and media composition on Cucumis and Cucurbita protoplast isolation and regeneration
- Factor affecting protoplast isolation and cultivation of Cucumis spp.
- Effect of UV radiation on mesophyl protoplasts of Cucumis sativus
- Gajdová, J., Lebeda, A., Navrátilová, B.: Effect of genotype, media composition and type of tissue on Cucumis and Cucurbita protoplast isolation and regeneration. (Acta Hort.731: 89-94, 2007
- Kopie: Greplová M., Navrátilová B., Vyvadilová M., Klíma M. 2004 Electrofusion and chemical fusion of protoplasts in selected species of genus Solanum, Cucumis and Brassica, Piešťany, SK, 24-25 november 2004, s. 70-73
- Elektrofúze a chemické fúze protoplastů vybraných druhů rodu Solanum, Cucumis a Brassica
- Navrátilová B., Greplová M., Vyvadilová M., Klíma M., Gajdová J., Skálová D. 2004: Electrofusion of protoplasts in selected vegetables of Brassica, Cucumis and Solanum genus. In: Fari M.G., Holb I.(Eds.): Book of Abstracts, 5th Symposium In vitro Culture
- Navrátilová B., Greplová M., Vyvadilová M., Klíma M., Gajdová J., Skálová D. 2004: Electrofusion of protoplasts in selected vegetables of Brassica, Cucumis and Solanum genus. In: Fari M.G., Holb I.(Eds.): Book of Abstracts, 5th Symposium In vitro Cultur
- Protoplast isolation and fusion between Brassica carinata Braun. and Brassica rapa L.
- Protoplast fusion by electric field at the model material S. tuberosum L.
- Effect of UV-C irradiation on mesophyll protoplasts of Cucumis sativus
- Methods of isolation of Cucumis sativus and C. melo pollen grains and their utilization in in vitro pollination
- Interspecific hybridization of Cucumis anguria L. var. longaculeatus J. H. Kirk. and C. zeyheri Sond. via embryo-rescue
- Embryo rescue of cucumber (Cucumis sativus), muskmelon (C. melo) and some wild Cucumis species (C. anguria, C. zeyheri, C. metuliferus)
- Optimizing culture for in vitro pollination and fertilization in Cucumis sativus and C. melo
- Skálová, D., Gasmanová, N., Lebeda, A., Navrátilová, B.2005.: Interspecific hybridization between Cucumis sativus and wild Cucumis species through embryo culture. Acta Biol. Cracoviensia Ser. Botanica 47 (Suppl. 1), 2005, p. 81.
- Electrofusion of protoplasts in selected vegetables of Brassica, Cucumis and Solanum genera
- Greplová M., Polzerová H., Kopecký D., Navrátilová B., Suchánková P., Švecová R., Domkářova J. 2006: Polyploidization and Somatic hybridization – An alternative of potato breeding, Piešťany, SK, 14-15 november, 2006, s.113-114
- Navrátilová B., Greplová M., Vyvadilová M., Klíma M., Gajdová J., Skálová D. 2006: Electrofusion of protoplasts in selected vegetables of Brassica, Cucumis and Solanum genera. Acta Horticulturae, 725, s.801 - 805
- Navrátilová B., Greplová M., Gajdová J., Skálová D. 2006: Somatic hybridization by genus Cucumis, Piešťany, SK, 14-15 november, 2006, s.125- 126
- Greplová M., Polzerová H., Navrátilová B. 2006: An introduction to study of asymmetric somatic hybridization, Piešťany, SK, 14-15 november, 2006, s.115-116
- Skálová D., Navrátilová B., Lebeda A. and Gasmanová N. 2006:Effect of colchicine application on interspecific hybridization between cucumber (Cucumis sativus) and wild Cucumis spp., XIXth International congres on sexual plant reproduction from gametes
- Skálová D., Lebeda A., Navrátilová B. 2004: Embryo and ovule cultures in Cucumis species and their utilization in interspecific hybridization.
- Acclimatization of the somatic hybrids in the Solanum genus
- A study of asymetric somatic hybridization in genus Solanum.
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| is http://linked.open...vavai/cep/projekt
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