| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Brassica carinata mesophyll and B. rapa hypocotyl protoplasts were used for fusing experiments using polyethylene glycol (PEG). Treatment with 30% PEG 6000 MW for 10 minutes was more effective, while combinations with 30% PEG 6000 MW/15 min, 30% PEG 4000 MW/15 min and 30% PEG 4000 MW for 10 minutes reduced viability of protoplasts and cell wall regeneration, cell division and production of microcolonies. Fusion frequency was about 25% with the treatment of 30% PEG 6000 MW/10 min. Cell colonies forming callus was observed after 14 days of cultivation on liquid B medium with addition of liquid C medium. Microcalli were subsequently transferred to solid E and F medium. Shoot regeneration from calli was induced via indirect organogenesis and somatic embryogenesis on MS hormon free medium. In total 58 calli were obtained from fusions between B. rapa 31/96 and B. carinata line 1; 14 calli produce shoots. According to the estimate and morfological diferencies was half shoots heterocaryonicals. This observat
- Brassica carinata mesophyll and B. rapa hypocotyl protoplasts were used for fusing experiments using polyethylene glycol (PEG). Treatment with 30% PEG 6000 MW for 10 minutes was more effective, while combinations with 30% PEG 6000 MW/15 min, 30% PEG 4000 MW/15 min and 30% PEG 4000 MW for 10 minutes reduced viability of protoplasts and cell wall regeneration, cell division and production of microcolonies. Fusion frequency was about 25% with the treatment of 30% PEG 6000 MW/10 min. Cell colonies forming callus was observed after 14 days of cultivation on liquid B medium with addition of liquid C medium. Microcalli were subsequently transferred to solid E and F medium. Shoot regeneration from calli was induced via indirect organogenesis and somatic embryogenesis on MS hormon free medium. In total 58 calli were obtained from fusions between B. rapa 31/96 and B. carinata line 1; 14 calli produce shoots. According to the estimate and morfological diferencies was half shoots heterocaryonicals. This observat (en)
- Mezofylové a hypokotylové protoplasty B. carinata a B. rapa byly použity pro fúzní experimenty s využitím polyetylén glykolu (PEG). Použití 30% PEG 6000 MW 10 minut bylo efektivnější než kombinace 30% PEG 600 MW 15 minut, 30% PEG 4000 MW 15 minut a 30% PEG 4000 MW 10 minut, které snižovaly životnost protoplastů a zabraňovaly regeneraci buněčné stěny a buněčnému dělení mikrokolonií. Frekvence fúzí byla okolo 25% při použití 30% PEG 6000 MW 10 minut. Ze získaných regenerantů bylo okolo 40 % analyzovaných vzorků pouze B.carinata x B.carinata fúzantů, zbylých 60 % byli fúzanti mezi B. carinata a B. rapa. (cs)
|
| Title
| - Protoplast isolation and fusion between Brassica carinata Braun. and Brassica rapa L.
- Protoplast isolation and fusion between Brassica carinata Braun. and Brassica rapa L. (en)
- Izolace a fúze protoplastů mezi Brassica carinata Braun. a Brassica rapa L. (cs)
|
| skos:prefLabel
| - Protoplast isolation and fusion between Brassica carinata Braun. and Brassica rapa L.
- Protoplast isolation and fusion between Brassica carinata Braun. and Brassica rapa L. (en)
- Izolace a fúze protoplastů mezi Brassica carinata Braun. a Brassica rapa L. (cs)
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| skos:notation
| - RIV/00027006:_____/07:2285!RIV08-MZE-00027006
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(QF4108), Z(MZE0002700602)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00027006:_____/07:2285
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - PEG; protoplasts; Brassica; fusion (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Agricultura Tropica et Subtropica
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Klíma, Miroslav
- Bechyně, M.
- Beránek, M.
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
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| is http://linked.open...avai/riv/vysledek
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