About: Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.

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About: Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis. Goto Sponge NotDistinct Permalink

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Attributes	Values
rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Projekt
rdfs:seeAlso	http://www.isvav.cz/projectDetail.do?rowId=IAA1163201
Description	The principal aims of the joint research are (a) to develop new ways of detecting of nucleic acids bases using transfer technique in connection with elimination and stripping voltammetry, (b) to study short oligonucleotides in dependence on their sequence and length and (c) to use elimination voltammetry with linear scan and electrochemical impedance spectroscopy in oligonucleotides and nucleic acids analysis. Analysis will be performed at mercury and solid electrodes. It is expected that the results obtained in this project will be utilized as a simple sensitive and selective analytical procedures for the determination of nucleic acids and their components. The advatage of these analytical procedures consists in the possibility to modify electrodes by nucleic acids and oligonucleotides. Obtained results will be applied in various fields of biophysics, biochemistry and molecular biology. (en) Nejdůležitější cíle grantového výzkumu jsou (a) vyvinout nové způsoby detekce bází nukleových kyselin pomocí přenosové techniky ve spojení s eliminační a rozpouštěcí voltametrií, (b) studovat krátké oligonukleotidy v závislosti na jejich sekvenci a délce a (c) použít eliminační voltametrii s lineární polarizací elektrody a elektrochemickou impedanční spektroskopii v analýze oligonukleotidů a nukleových kyselin. Analýza bude provedena na rtuťových a pevných elektrodách. Předpokládá se, že výsledky získané v tomto grantu budou využity jako jednoduché, citlivé a selektivní analytické postupy stanovení nukleových kyselin a jejich složek. Výhoda těchto analytických postupů spočívá v možnosti modifikovat elektrody nukleovými kyselinami a oligonukleotidy. Získané výsledky najdou uplatnění v různých oblastech biofyziky, biochemie a molekulární biologie.
Title	Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis. (en) Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin
skos:notation	IAA1163201
http://linked.open...avai/cep/aktivita	Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the Academy of Sciences of the Czech Republic
http://linked.open...kovaStatniPodpora	http://linked.opendata.cz/resource/domain/vavai/projekt/IAA1163201/celkovaStatniPodpora
http://linked.open...ep/celkoveNaklady	http://linked.opendata.cz/resource/domain/vavai/projekt/IAA1163201/celkoveNaklady
http://linked.open...datumDodatniDoRIV	2007-09-26 (xsd:date)
http://linked.open...i/cep/druhSouteze	VS - Veřejná soutěž ve výzkumu a vývoji
http://linked.open...ep/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open.../cep/fazeProjektu	1717753
http://linked.open...ai/cep/hlavniObor	BO - Biofyzika
http://linked.open...hodnoceniProjektu	V - Vynikající výsledky (s mezinárodním významem apod.). Zároveň byly splněny cíle a předpokládané výsledky uvedené ve smlouvě / rozhodnutí o poskytnutí podpory.
http://linked.open...vai/cep/kategorie	ZV - Základní výzkum
http://linked.open.../cep/klicovaSlova	oligonucleotides; nucleic acids; adsorptive transfer technique; elimination voltammetry nith linear scan; mercury and solid electrodes; biosensors (en)
http://linked.open...ep/partnetrHlavni	Přírodovědecká fakulta
http://linked.open...inujicichPrijemcu	0 (xsd:int)
http://linked.open...cep/pocetPrijemcu	2 (xsd:int)
http://linked.open...ocetSpoluPrijemcu	0 (xsd:int)
http://linked.open.../pocetVysledkuRIV	57 (xsd:int)
http://linked.open...enychVysledkuVRIV	57 (xsd:int)
http://linked.open...okUkonceniPodpory	2004
http://linked.open...okZahajeniPodpory	2002
http://linked.open...iciPoslednihoRoku	2005
http://linked.open...atUdajeProjZameru	2005
http://linked.open.../vavai/cep/soutez	SAV02002-AB
http://linked.open...usZobrazovaneFaze	DUU
http://linked.open...ai/cep/typPojektu	P - Projekt výzkumu a vývoje financovaný ze státního rozpočtu
http://linked.open.../cep/vedlejsiObor	CF - Fyzikální chemie a teoretická chemie CG - Elektrochemie
http://linked.open...jektu+dodavatelem	Elimination voltammetry with linear scan in combination with adsorptive stripping technique was developed as a perspective tool for the sensitive electroanalytical determ. of homo- and hetero-oligonucleotides, for the estimation of nucleotide sequence (en) Kombinací adsorptivní rozpouštěcí techniky a eliminační voltametrie s lineárním scanem byl vytvořen perspektivní nástroj pro citlivé analytické stanovení homo- i hetero-oligonukleotidů, včetně jejich bází a pro určení jejich nukleotidové sekvence. (cs)
http://linked.open...tniCyklusProjektu	ZBKU
http://linked.open.../cep/klicoveSlovo	oligonucleotides adsorptive transfer technique elimination voltammetry nith linear scan mercury and solid electrodes nucleic acids
is http://linked.open...vavai/riv/projekt of	An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques Current elimination coefficient beta Description of binary system by artificial neural networks Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED) Cyclic voltammetric study of the redox system of glutathione using the disulfide bond reductant tris(2-carboxyethyl)phosphine Determination of azidothymidine - an antiproliferative and virostatic drug by square-wave voltammetry Microanalysis of DNA by stripping transfer voltammetry Study of the influence of boric acid on the Cd2+ reduction process by elimination voltammetry with linear scan The protonation/deprotonation equilibrium of azido-analogues of thymidine and uridine studied by UV spectroscopy and chemometrics 4th Workshop of Physical Chemists and Electrochemists Determination of vitamin C by performance liquid chromatography coupled with electrochemical detector Studium vlivu těžkých kovů na růst baktérií Stanovení isoflavonů pomocí vysoko-účinné kapalinové chromatografie s elektrochemickou detekcí Square wave and elimination voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA Study of relationship between metallothionein and heavy metals by chronopotentiometry stripping analysis Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutatione in the maize (Zea mays L.) kernels exposed to cadmium Study of the reduction of cadmium on various electrode-electrolyte interfaces by elimination voltammetry with linear scan Stripping voltammetry of oligonucleotides Use of artificial neural networks for the evaluation UV-Vis spectra of adenine and cytosine in mixtures Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides. I. Homo-oligodeoxynucleotides dA9 and dC9 Application of elimination voltammetry with linear scan in bioelectrochemistry Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize. Elimination voltammetry of oligonucleotides Generalized principle of elimination voltammetry Elektrochemické stanovení těžkých kovů ve složité biologické matrici Electroanalytical determination of d(GCGAAGC) hairpin Elimination voltammetry of oligonucleotides Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode Electrochemical determination of lead and glutathione in a plant cell culture Label-free determination of picogram quantities of DNA by stripping voltammetry with solid copper amalgam or mercury electrodes in the presence of copper. Electrochemical detection of chromosomal translocation T (15;17) as an acute promyelotic leukemie marker Electrochemical determination of d(GCGAAAGC) hairpin Electrochemical determination of glutathion and phytochelatins redox state Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica Electrochemical biosensors in agricultural and enviromental analysis Elimination Voltammetry of Nucleic Acids on Silver Electrodes Electrochemical behavior of DNA at a silver electrode studied by cyclic and elimination voltammetry Elimination voltammetry of short oligonucleotides Partial least squares and artificial neural networks for multicomponent analysis from derivative UV-Vis spectra Advantages of Elimination Voltammetry Využití elektrochemických metod pro stanovení metalothioneinu a fytochelatinu Využití avidin-biotinové technologie pro elektrochemickou detekci nukleových kyselin Utilization of elimination voltammetry for the study of reduction of cadmium at various electrodes Utilization of catalytic reactions on mercury electrode for the electrochemical determination of metalothioneins Application of catalytic reactions on a mercury electrode for electrochemical detection of metallothioneins Square wave voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA Square-wave voltammetric determination of cefoperazone in a bacterial culture, pharmaceutical drug, milk, and urine. Verifikácia teórie eliminačnej voltampérometrie na tuhej grafitovej elektróde The resolution of adenine and cytosine signals in oligonucleotides by means of elimination voltammetry Study of electroplating processes on the graphite electrode using elimination voltammetry Analýza thiolových sloučenin pomocí vysoko-učinné kapalinové chromatografie s elektrochemickou detekcí Application of elimination voltammetry with linear scan to the study of reduction of cadmium on various electrodes Application of elimination voltammetry in the study of electroplating processes on the graphite electrode Application of elimination voltammetry with linear scan to the study of electrochemical reactions of metal ions on various electrodes Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel electrochemical detector in online connection with reversed-phase liquid chromatography The protonation/deprotonation equilibrium of thymidine and azidothymidine studied by UV spectra and multivariate curve resolution
is http://linked.open...vavai/cep/projekt of	http://linked.opendata.cz/resource/domain/vavai/cep/ucast/IAA1163201/2002/ico%3A68081707 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/IAA1163201/2002/orjk%3A14310

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