About: Electrochemical determination of lead and glutathione in a plant cell culture

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this work we established differential pulse anodic stripping voltammetry (DPASV) as the tool for analysis of lead in the plant cell culture. For the cultivation procedure, lead in Pb(II)-ethylenediaminetetraacetic acid (Pb-EDTA) chelate has been used. The detection limit of lead was found at 500 pM in phosphate buffer (pH 5.5), and 100 nM in prepared cells intracellular extract (20 pg Pb(II)/mg cells). For determination of cysteine-rich peptides, voltammetry in differential mode (DPV) in cobalt(Ill)-containing ammonia buffer (Brdicka reaction) was used. In this short communication, we present suitable voltammetric techniques for the physiological study of lead and thiols in plant cell culture. In this work we established differential pulse anodic stripping voltammetry (DPASV) as the tool for analysis of lead in the plant cell culture. For the cultivation procedure, lead in Pb(II)-ethylenediaminetetraacetic acid (Pb-EDTA) chelate has been used. The detection limit of lead was found at 500 pM in phosphate buffer (pH 5.5), and 100 nM in prepared cells intracellular extract (20 pg Pb(II)/mg cells). For determination of cysteine-rich peptides, voltammetry in differential mode (DPV) in cobalt(Ill)-containing ammonia buffer (Brdicka reaction) was used. In this short communication, we present suitable voltammetric techniques for the physiological study of lead and thiols in plant cell culture. (en) Pro stanovení olova v buněčné kultuře rostlin byla použita diferenční pulzní anodická rozpouštěcí voltametrie. Pro kultivační postupy bylo použito olovo ve formě chelátu Pb-EDTA. Detekční limit olova byl nalezen při koncentraci 500 nM ve fosfátovém pufru, pH 5,5, nebo 100 nM v intracelulárním buněčném extraktu (20 pg/Pb(II)/mg buněk). Pro stanovení peptidů obsahujících větší množství cysteinu byla použita diferenční pulzní voltametrie (DPV) v prostředí kobaltu(II) s amoniakálním pufrem (Brdičkova reakce). V této studii je ukázáno, že voltametrické metody jsou vhodné pro fyziologické analýzy, při kterých se monitoruje množství olova i thiolů v buněčných kulturách rostlin. (cs)
Title	Electrochemical determination of lead and glutathione in a plant cell culture Elektrochemické stanovení olova a glutationu v buněčné kultuře rostlin (cs) Electrochemical determination of lead and glutathione in a plant cell culture (en)
skos:prefLabel	Electrochemical determination of lead and glutathione in a plant cell culture Elektrochemické stanovení olova a glutationu v buněčné kultuře rostlin (cs) Electrochemical determination of lead and glutathione in a plant cell culture (en)
skos:notation	RIV/68081707:_____/04:00104666!RIV/2005/AV0/A17005/N
http://linked.open.../vavai/riv/strany	347;351
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/02/0422), P(IAA1163201), Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Vacek, Jan Jelen, František Kizek, René
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	562507
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/04:00104666
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	determination of lead; Pb(II)-EDTA; voltammetry (en)
http://linked.open.../riv/klicoveSlovo	determination of lead Pb(II)-EDTA voltammetry
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[C872419205D9]
http://linked.open...i/riv/nazevZdroje	Bioelectrochemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	63
http://linked.open...iv/tvurceVysledku	Kizek, René Vacek, Jan Jelen, František Havel, L. Klejdus, B. Trnková, L. Petřek, J.
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors
issn	1567-5394
number of pages	5 (xsd:int)

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