About: Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain

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About: Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The Bordetella adenylate cyclase toxin-hemolysin (CyaA; also called ACT or AC-Hly) targets CD11b-expressing phagocytes and translocates into their cytosol an adenylyl cyclase (AC) that hijacks cellular signaling by conversion of ATP to cyclic AMP (cAMP). Intriguingly, insertion of large passenger peptides removes the enzymatic activity but not the cell-invasive capacity of the AC domain. This has repeatedly been exploited for delivery of heterologous antigens into the cytosolic pathway of CD11b-expressing dendritic cells by CyaA/AC(-) toxoids, thus enabling their processing and presentation on major histocompatibility complex (MHC) class I molecules to cytotoxic CD8(+) T lymphocytes (CTLs). We produced a set of toxoids with overlapping deletions within the first 371 residues of CyaA and showed that the structure of the AC enzyme does not contain any sequences indispensable for its translocation across target cell membrane The Bordetella adenylate cyclase toxin-hemolysin (CyaA; also called ACT or AC-Hly) targets CD11b-expressing phagocytes and translocates into their cytosol an adenylyl cyclase (AC) that hijacks cellular signaling by conversion of ATP to cyclic AMP (cAMP). Intriguingly, insertion of large passenger peptides removes the enzymatic activity but not the cell-invasive capacity of the AC domain. This has repeatedly been exploited for delivery of heterologous antigens into the cytosolic pathway of CD11b-expressing dendritic cells by CyaA/AC(-) toxoids, thus enabling their processing and presentation on major histocompatibility complex (MHC) class I molecules to cytotoxic CD8(+) T lymphocytes (CTLs). We produced a set of toxoids with overlapping deletions within the first 371 residues of CyaA and showed that the structure of the AC enzyme does not contain any sequences indispensable for its translocation across target cell membrane (en)
Title	Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain (en)
skos:prefLabel	Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain (en)
skos:notation	RIV/61388971:_____/12:00378997!RIV13-GA0-61388971
http://linked.open...avai/predkladatel	Mikrobiologický ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0506), P(2B06161), P(GAP207/11/0717), P(GAP301/11/0325), P(IAA500200914), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Kovář, Marek Tomala, Jakub Šebo, Peter Kamanová, Jana Mašín, Jiří Holubová, Jana Kosová, Martina Staněk, Ondřej Bumba, Ladislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	129984
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/12:00378997
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MHC CLASS-I; ESCHERICHIA-COLI; PRESENTATION PATHWAY (en)
http://linked.open.../riv/klicoveSlovo	ESCHERICHIA-COLI MHC CLASS-I PRESENTATION PATHWAY
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[B75B6E49C098]
http://linked.open...i/riv/nazevZdroje	Infection and Immunity
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	9 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular mechanism of adenylate cyclase toxin penetration into phagocytes Molecular mechanism of autocatalytic protein splicing of the RTX protein FrpC from Neisseria meningitidis Center of Molecular and Cellular Immunology New tools for rapid and non-invasive diagnosing of latent infections. Depletion of T regulatory cells without need of anti-CD25 mAb for effective chemoimmunotherapy by HPMA-based polymeric drugs and IL-2 immunocomplexes
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	80
http://linked.open...iv/tvurceVysledku	Holubová, Jana Kovář, Marek Tomala, Jakub Kamanová, Jana Šebo, Peter Michálek, J. Jelínek, J. Kosová, Martina Bumba, Ladislav Mašín, Jiří Staněk, Ondřej
http://linked.open...ain/vavai/riv/wos	000300621600031
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	0019-9567
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1128/IAI.05711-11
is http://linked.open...avai/riv/vysledek of	Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain

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