About: Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels.

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About: Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The haloalkane dehalogenase DhaA from Rhodococcus rhodochrous NCIMB 13064 is a bacterial enzyme that shows catalytic activity for the hydrolytic degradation of the highly toxic industrial pollutant 1,2,3-trichloropropane (TCP). Mutagenesis focused on the access tunnels of DhaA produced protein variants with significantly improved activity towards TCP. Three mutants of DhaA named DhaA04 (C176Y), DhaA14 (I135F) and DhaA15 (C176Y + I135F) were constructed in order to study the functional relevance of the tunnels connecting the buried active site of the protein with the surrounding solvent. All three protein variants were crystallized using the sitting-drop vapour-diffusion technique. The haloalkane dehalogenase DhaA from Rhodococcus rhodochrous NCIMB 13064 is a bacterial enzyme that shows catalytic activity for the hydrolytic degradation of the highly toxic industrial pollutant 1,2,3-trichloropropane (TCP). Mutagenesis focused on the access tunnels of DhaA produced protein variants with significantly improved activity towards TCP. Three mutants of DhaA named DhaA04 (C176Y), DhaA14 (I135F) and DhaA15 (C176Y + I135F) were constructed in order to study the functional relevance of the tunnels connecting the buried active site of the protein with the surrounding solvent. All three protein variants were crystallized using the sitting-drop vapour-diffusion technique. (en)
Title	Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels. Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels. (en)
skos:prefLabel	Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels. Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels. (en)
skos:notation	RIV/00216224:14310/10:00045039!RIV13-MSM-14310___
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GA310/09/1407), P(LC06010), S, Z(AV0Z40500505), Z(AV0Z60870520), Z(MSM0021622412), Z(MSM0021622413), Z(MSM6007665808)
http://linked.open...iv/cisloPeriodika	66
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Koudeláková, Táňa Kutá-Smatanová, Ivana Kutý, Michal Damborský, Jiří Stsiapanava, Alena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	248048
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/10:00045039
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	haloalkane dehalogenase DhaA; Rhodococcus rhodochrous NCIMB 13064; hydrolytic degradation; 1.2.3-trichloropropane (TCP) (en)
http://linked.open.../riv/klicoveSlovo	1.2.3-trichloropropane (TCP) Rhodococcus rhodochrous NCIMB 13064 haloalkane dehalogenase DhaA hydrolytic degradation
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[991769FFFFC0]
http://linked.open...i/riv/nazevZdroje	ACTA CRYSTALLOGRAPHICA D
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Chitinolytic enzymes from Clostridium paraputrificum inhabiting the human gastrointestinal tract; structure and biomedical implications. Center of Biocatalysis and Biotransformation
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	66
http://linked.open...iv/tvurceVysledku	Damborský, Jiří Kutá-Smatanová, Ivana Koudeláková, Táňa Stsiapanava, Alena Kutý, Michal Dohnálek, J. Gavira, J.
http://linked.open...ain/vavai/riv/wos	000281635500002
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Spatial and functional dynamics of biological, ecological and socio-economic systems interacting with global climatic change Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Proteiny v metabolismu a při interakci organismů s prostředím Fyzikální biologie -nové přístupy v biologickém výzkumu
issn	0907-4449
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14310
is http://linked.open...avai/riv/vysledek of	Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels.

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