About: Role of individual phosphorylation sites for the 14-3-3-protein-dependent activation of yeast neutral trehalase Nth1

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this work, we investigated in detail the interaction between Saccharomyces cerevisiae Nth1 and 14-3-3 protein isoforms Bmh1 and Bmh2. We determined four residues that are phosphorylated by PKA within the disordered N-terminal segment of Nth1. Sedimentation analysis and enzyme kinetics measurements show that both yeast 14-3-3 isoforms form a stable complex with phosphorylated NTH1 and significantly enhance its enzyme activity. Site-directed mutagenesis was used to decipher the importance of found phosphorylation sites for Nth1 activation. These experiments suggest that Ser60 and Ser83 are essential sites for PKA-dependent and 14-3-3-mediated activation of Nth1. The phosphorylation of either of these sites is sufficient for full 14-3-3-dependent activation of Nth1 In this work, we investigated in detail the interaction between Saccharomyces cerevisiae Nth1 and 14-3-3 protein isoforms Bmh1 and Bmh2. We determined four residues that are phosphorylated by PKA within the disordered N-terminal segment of Nth1. Sedimentation analysis and enzyme kinetics measurements show that both yeast 14-3-3 isoforms form a stable complex with phosphorylated NTH1 and significantly enhance its enzyme activity. Site-directed mutagenesis was used to decipher the importance of found phosphorylation sites for Nth1 activation. These experiments suggest that Ser60 and Ser83 are essential sites for PKA-dependent and 14-3-3-mediated activation of Nth1. The phosphorylation of either of these sites is sufficient for full 14-3-3-dependent activation of Nth1 (en)
Title	Role of individual phosphorylation sites for the 14-3-3-protein-dependent activation of yeast neutral trehalase Nth1 Role of individual phosphorylation sites for the 14-3-3-protein-dependent activation of yeast neutral trehalase Nth1 (en)
skos:prefLabel	Role of individual phosphorylation sites for the 14-3-3-protein-dependent activation of yeast neutral trehalase Nth1 Role of individual phosphorylation sites for the 14-3-3-protein-dependent activation of yeast neutral trehalase Nth1 (en)
skos:notation	RIV/67985823:_____/12:00376829!RIV13-GA0-67985823
http://linked.open...avai/predkladatel	Fyziologický ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GAP207/11/0455), P(IAA500110801), S, Z(AV0Z50110509), Z(AV0Z50200510), Z(MSM0021620857)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Sychrová, Hana Obšilová, Veronika Řežábková, Lenka Macáková, Eva Veisová, Dana Vácha, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	166009
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/12:00376829
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	14-3-3 protein; Bmh; neutral trehalase (Nth1); enzymatic activity; phosphorylation; Saccharomyces cerevisiae (en)
http://linked.open.../riv/klicoveSlovo	Saccharomyces cerevisiae enzymatic activity phosphorylation 14-3-3 protein Bmh neutral trehalase (Nth1)
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[5BBAABD80D4C]
http://linked.open...i/riv/nazevZdroje	Biochemical Journal
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Role of BMH proteins in the regulation of yeast enzyme neutral trehalase Role of Na/H antiporters in cell physiology - transporters teamwork in intracellular pH and K+ homeostasis
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	443
http://linked.open...iv/tvurceVysledku	Sychrová, Hana Šulc, Miroslav Obšil, T. Obšilová, Veronika Macáková, Eva Veisová, Dana Vácha, Petr Řežábková, Lenka
http://linked.open...ain/vavai/riv/wos	000303944200008
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka Microorganisms in Research and Biotechnology Nové molekulární systémy pro pokročilé aplikace prospěšné pro zdraví a šetrné k životnímu prostředí
issn	0264-6021
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1042/BJ20111615
is http://linked.open...avai/riv/vysledek of	Role of individual phosphorylation sites for the 14-3-3-protein-dependent activation of yeast neutral trehalase Nth1

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