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| rdf:type
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| Description
| - Activity of LFA-1 (CD11a/CD18) must be tightly controlled at onset of cellular immunity. Sialoglycoprotein CD43 can affect LFA-1-mediated cell adhesion in anti- or pro-adhesive manner. Using Y2H screen and coimmunoprecipitation we identified and characterized physical and functional association of CD43 with 2 novel partners, LFA-1 and CD147. Monoclonal antibodies to both CD43 and CD147 induced homotypic cell aggregation and adhesion of Jurkat T cells and U937 myeloid cells as well as dynamic co-capping of LFA-1 together with CD43 and CD147 to cell contact zones. Cotransfection of CD43 interfered with CD147-induced cell adhesion and aggregation, and siRNA-mediated knock down of CD43 in human T cells led to enhanced LFA-1 activation induced via CD147 and T cell antigen receptor. Triggering CD43 and underlying signaling pathways thus seems to enhance LFA-1 adhesiveness. CD43 also negatively regulates LFA-1 induction via other receptors by dynamic interaction with either LFA-1 or CD147.
- Activity of LFA-1 (CD11a/CD18) must be tightly controlled at onset of cellular immunity. Sialoglycoprotein CD43 can affect LFA-1-mediated cell adhesion in anti- or pro-adhesive manner. Using Y2H screen and coimmunoprecipitation we identified and characterized physical and functional association of CD43 with 2 novel partners, LFA-1 and CD147. Monoclonal antibodies to both CD43 and CD147 induced homotypic cell aggregation and adhesion of Jurkat T cells and U937 myeloid cells as well as dynamic co-capping of LFA-1 together with CD43 and CD147 to cell contact zones. Cotransfection of CD43 interfered with CD147-induced cell adhesion and aggregation, and siRNA-mediated knock down of CD43 in human T cells led to enhanced LFA-1 activation induced via CD147 and T cell antigen receptor. Triggering CD43 and underlying signaling pathways thus seems to enhance LFA-1 adhesiveness. CD43 also negatively regulates LFA-1 induction via other receptors by dynamic interaction with either LFA-1 or CD147. (en)
- Aktivita LFA-1 (CD11a/CD18) musí být při nástupu buněčné imunity důsledně kontrolována. Sialoglykoprotein CD43 může pozitivně či negativně ovlivnit LFA-1-zprostředkovanou buněčnou adhezi. Pomocí Y2H vyhledávání a koimunoprecipitace jsme identifikovali a charakterizovali fyzikální a funkční interakci CD43 se 2 novými partnery, LFA-1 a CD147. Monoklonální protilátky proti CD43 a CD147 indukovaly homotypickou agregaci a adhezi Jurkat T a U937 myeloidních buněk i dynamickou kolokalizaci LFA-1 spolu s CD43 a CD147 na kontaktních zónách buněk. Kotransfekce CD43 interferovala s CD147-indukovanou buněčnou adhezí a agregace a siRNA-zprostředkované potlačení exprese CD43 v lidských T buňkách vedlo ke zvýšené aktivaci LFA-1 indukované přes CD147 a T buněčný receptor. Aktivace CD43 a s ním spojených signálních drah tedy zřejmě zlepšuje adhezivní vlastnosti LFA-1. CD43 ale také negativně reguluje indukci LFA-1 skrze jiné receptory a to dynamickou interakcí s LFA-1 či s CD147. (cs)
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| Title
| - LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147
- LFA-1-zprostředkovaná adheze leukocytů je regulována interakcemi CD43 s LFA-1 a CD147 (cs)
- LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147 (en)
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| skos:prefLabel
| - LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147
- LFA-1-zprostředkovaná adheze leukocytů je regulována interakcemi CD43 s LFA-1 a CD147 (cs)
- LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147 (en)
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| skos:notation
| - RIV/68378050:_____/08:00307370!RIV09-AV0-68378050
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(1M0506), Z(AV0Z50520514)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/08:00307370
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - leukocyte adhesion and aggregation; monoclonal antibodies; receptor signaling (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Anděra, Ladislav
- Hořejší, Václav
- Čermák, Lukáš
- Khunkaewla, P.
- Leksa, V.
- Paster, W.
- Schiller, H. B.
- Stockinger, H.
|
| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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