About: Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Flavoprotein WrbA z Escherichia coli je považován za prototyp nové rodiny multimerních proteinů flavodoxinového typu zapojených do obrany buňky proti oxidačnímu stresu. Tato studie je zaměřena na charakterizaci struktury proteinu E. coli s ohledem na jeho nedávno zjištěnou oxidoreduktázovou aktivitu. Krystaly holoproteinu WrbA v komplexu s oxidovaným flavinovým kofaktorem (FMN) byly získány standardními technikami difúze par. Temně žluté tetragonální krystaly získané za odlišných krystalizačních podmínek vykazují různé parametry prostorového uspořádání. Rentgenová struktura holoproteinu WrbA byla určena při rozlišení 2.0 a 2.6 A (cs) The flavoprotein WrbA from Escherichia coli is considered to be the prototype of a new family of multimeric flavodoxin-like proteins that are implicated in cell protection against oxidative stress. The present study is aimed at structural characterization of the E. coli protein with respect to its recently revealed oxidoreductase activity. Crystals of WrbA holoprotein in complex with the oxidized flavin cofactor (FMN) were obtained using standard vapour-diffusion techniques. Deep yellow tetragonal crystals obtained from differing crystallization conditions display different space groups and unit-cell parameters. X-ray crystal structures of the WrbA holoprotein have been determined to resolutions of 2.0 and 2.6 A. The flavoprotein WrbA from Escherichia coli is considered to be the prototype of a new family of multimeric flavodoxin-like proteins that are implicated in cell protection against oxidative stress. The present study is aimed at structural characterization of the E. coli protein with respect to its recently revealed oxidoreductase activity. Crystals of WrbA holoprotein in complex with the oxidized flavin cofactor (FMN) were obtained using standard vapour-diffusion techniques. Deep yellow tetragonal crystals obtained from differing crystallization conditions display different space groups and unit-cell parameters. X-ray crystal structures of the WrbA holoprotein have been determined to resolutions of 2.0 and 2.6 A. (en)
Title	Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide Krystalizace a předběžná difrakční analýza WrbA z E. coli v komplexu s jeho kofaktorem flavin mononukleotidem. (cs) Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide (en)
skos:prefLabel	Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide Krystalizace a předběžná difrakční analýza WrbA z E. coli v komplexu s jeho kofaktorem flavin mononukleotidem. (cs) Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide (en)
skos:notation	RIV/68378050:_____/07:00097985!RIV08-AV0-68378050
http://linked.open.../vavai/riv/strany	571;575
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(LC06010), P(ME 640), S, Z(AV0Z50520514), Z(AV0Z60870520), Z(MSM6007665808)
http://linked.open...iv/cisloPeriodika	Pt7
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Brynda, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	415366
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/07:00097985
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	WrbA; flavodoxin; crystal structure (en)
http://linked.open.../riv/klicoveSlovo	flavodoxin crystal structure WrbA
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[0AA09D509FF9]
http://linked.open...i/riv/nazevZdroje	Acta Crystallographica Section F
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of Biocatalysis and Biotransformation http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20640
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	63
http://linked.open...iv/tvurceVysledku	Brynda, Jiří Kutá-Smatanová, Ivana Carey, J. Wolfová, Julie Mesters, J. R. Grandori, R. Natalello, A.
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů Spatial and functional dynamics of biological, ecological and socio-economic systems interacting with global climatic change Fyzikální biologie -nové přístupy v biologickém výzkumu
issn	1744-3091
number of pages	5 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide Crystallization and preliminary diffraction analysis of Escherichia coli WrbA in complex with its cofactor flavin mononucleotide

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