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Description
| - The Hybrid sterility 1 (Hst1) gene affects fertility of male hybrids between certain laboratory strains (such as C57BL/10) and some Mus musculus musculus mice by causing a breakdown of spermatogenesis at the stage of primary spermatocytes. In the process of positional cloning of the Hst1 gene, we generated a contig of bacterial artificial chromosomes and subsequently a low coverage sequence of the candidate region of the 129S1/SvImJ strain. Development of new genetic markers allowed us to narrow the Hst1 region from 580 to 360 kb. The products of two genes from this region, TATA-binding protein (Tbp) and proteasome subunit beta 1 (Psmb1), accumulate during spermatogenesis. These proteins have been described previously as having conserved C-terminal sequences and species-specific N-termini. We evaluated the candidacy of these genes for Hst1 by allelic sequencing and by real-time semiquantitative reverse-transcription PCR of testicular mRNAs.
- The Hybrid sterility 1 (Hst1) gene affects fertility of male hybrids between certain laboratory strains (such as C57BL/10) and some Mus musculus musculus mice by causing a breakdown of spermatogenesis at the stage of primary spermatocytes. In the process of positional cloning of the Hst1 gene, we generated a contig of bacterial artificial chromosomes and subsequently a low coverage sequence of the candidate region of the 129S1/SvImJ strain. Development of new genetic markers allowed us to narrow the Hst1 region from 580 to 360 kb. The products of two genes from this region, TATA-binding protein (Tbp) and proteasome subunit beta 1 (Psmb1), accumulate during spermatogenesis. These proteins have been described previously as having conserved C-terminal sequences and species-specific N-termini. We evaluated the candidacy of these genes for Hst1 by allelic sequencing and by real-time semiquantitative reverse-transcription PCR of testicular mRNAs. (en)
- Gen Hybrid sterility 1 (Hst1) ovlivňuje fertilitu hybridních samců mezi některými laboratorními kmeny (např. C57BL/10) a myší (pod)druhu Mus musculus musculus. Během pozičního klonování Hst1 genu jsme vygenerovali kontig bakteriálních artificiálních chromosomů (BAC) a vzorkovou sekvenci kandidátní oblasti ze 129S1/SvImJ kmene. Nové genetické markery nám umožnily zúžit Hst1 oblast z 580 na 360 kb. Produkty dvou genů z této oblasti, TATA-vazebný protein (Tbp) a proteasomová podjednotka beta 1 (Psmb1), akumulují během spermatogeneze. Tyto proteiny mají konzervované C-terminalní sekvence a druhově-specifické N-konce. Vyhodnotili jsme kandidaturu těchto genů na gen Hst1 alelickým sekvenováním a semikvantitativním RT PCR v reálném čase pro jejich testikulární mRNA. (cs)
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Title
| - Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes
- Poziční klonování genu Hybrid sterility 1: genetické mapování a evaluace dvou kandidátních genů (cs)
- Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes (en)
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skos:prefLabel
| - Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes
- Poziční klonování genu Hybrid sterility 1: genetické mapování a evaluace dvou kandidátních genů (cs)
- Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes (en)
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skos:notation
| - RIV/68378050:_____/05:00000229!RIV/2005/AV0/A23005/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA204/01/0997), P(GV204/98/K015), P(LN00A079), Z(AV0Z50520514), Z(AV0Z5052915)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/05:00000229
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - house mouse; Mus musculus; speciation (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Biological Journal of the Linnean Society
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Forejt, Jiří
- Pačes, Václav
- Vlček, Čestmír
- Trachtulec, Zdeněk
- Mihola, Ondřej
- Himmelbauer, H.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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is http://linked.open...avai/riv/vysledek
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