About: Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR White embedding of HeLa cells     Goto   Sponge   NotDistinct   Permalink

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  • This article presents new protocol for high pressure freezing and freeze substitution of mammalian cells grown in suspension suitable for immuno-electron microscopy. It shows the suitability of LR White resin usage for ultrastructure and antigen preservation of cells that were not chemically fixed.
  • This article presents new protocol for high pressure freezing and freeze substitution of mammalian cells grown in suspension suitable for immuno-electron microscopy. It shows the suitability of LR White resin usage for ultrastructure and antigen preservation of cells that were not chemically fixed. (en)
  • Článek popisuje nový protokol pro přípravu suspenzních savčích buněk pro imuno-elektronovou mikroskopii metodami vysokotlakého zamražování a mrazové substituce. Je zde dokumentováno, že pryskyřice LR White je vhodná pro zachování dobré ultrastruktury a epitopů pro imunoznačení u buněk, které nebyly chemicky fixovány. (cs)
Title
  • Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR White embedding of HeLa cells
  • Ultrastruktura a detekce jaderných antigenů v HeLa buňkách zalitých do LR White po vysokotlakém zamražení a mrazové substituci (cs)
  • Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR White embedding of HeLa cells (en)
skos:prefLabel
  • Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR White embedding of HeLa cells
  • Ultrastruktura a detekce jaderných antigenů v HeLa buňkách zalitých do LR White po vysokotlakém zamražení a mrazové substituci (cs)
  • Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR White embedding of HeLa cells (en)
skos:notation
  • RIV/68378041:_____/08:00315843!RIV09-AV0-68378041
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(2B06063), P(GD204/05/H023), P(KAN200520704), P(LC545), Z(AV0Z50390512), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika
  • -
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 401322
http://linked.open...ai/riv/idVysledku
  • RIV/68378041:_____/08:00315843
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Cryofixation; Freeze substitution; High-presszre freezing (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [699FA32CCACC]
http://linked.open...i/riv/nazevZdroje
  • Histochemistry and Cell Biology
http://linked.open...in/vavai/riv/obor
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 130
http://linked.open...iv/tvurceVysledku
  • Hozák, Pavel
  • Strádalová, Vendula
  • Kyselá, Katarína
http://linked.open...ain/vavai/riv/wos
  • 259997900020
http://linked.open...n/vavai/riv/zamer
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  • 0948-6143
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