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Description
  • Open tubular capillary enzyme reactors were studied for rapid protein digestion. TPCK-trypsin and pepsin A were immobilized directly on the inside wall of a 10 micrometer I.D. capillary and the optimized reactors were tested as the nanospray needles for fast identification of proteins using CE-ESI/TOF-MS.
  • Open tubular capillary enzyme reactors were studied for rapid protein digestion. TPCK-trypsin and pepsin A were immobilized directly on the inside wall of a 10 micrometer I.D. capillary and the optimized reactors were tested as the nanospray needles for fast identification of proteins using CE-ESI/TOF-MS. (en)
  • Práce se zabývá využitím otevřených kapilárních reaktorů s imobilizovanými enzymy pro štěpení proteinů. TPCK-trypsin a pepsin A byly imobilizovány na stěnu křemenné kapiláry o vnitřním průměru 10 mikrometrů a optimalizované reaktory byly testovány jako nanosprejové špičky pro identifikaci proteinů pomocí CE-ESI/TOF-MS. (cs)
Title
  • Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries
  • Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries (en)
  • Spojení kapilární elektroforézy a hmotnostní spektrometrie pomocí sprejovacích kapilár s imobilizovanými enzymy (cs)
skos:prefLabel
  • Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries
  • Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries (en)
  • Spojení kapilární elektroforézy a hmotnostní spektrometrie pomocí sprejovacích kapilár s imobilizovanými enzymy (cs)
skos:notation
  • RIV/68081715:_____/07:00084856!RIV08-AV0-68081715
http://linked.open.../vavai/riv/strany
  • 110;118
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA203/06/1685), P(IAA400310506), P(KAN400310651), P(KJB400310604), P(LC06023), Z(AV0Z40310501)
http://linked.open...iv/cisloPeriodika
  • 1-2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 412602
http://linked.open...ai/riv/idVysledku
  • RIV/68081715:_____/07:00084856
http://linked.open...riv/jazykVysledku
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  • capillary enzyme reactor; electrospray; capillary electrophoresis mass spectrometry coupling (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [480247C3D050]
http://linked.open...i/riv/nazevZdroje
  • Journal of Chromatography A
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 1159
http://linked.open...iv/tvurceVysledku
  • Foret, František
  • Klepárník, Karel
  • Křenková, Jana
http://linked.open...n/vavai/riv/zamer
issn
  • 0021-9673
number of pages
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