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| Description
| - The goal of the project is basic research of nanostructured surfaces for electronic detection of biochemical interactions. The experience and know-how of the partner laboratories will open up a new research direction based on original methods for analysis of DNA and proteins combining nanotechnology, microfluidics and electrochemistry. The research of the nanostructures will be directed towards surface enhanced analytical performance using nanoporous substrates, magnetic particles and carbon nanotubes. Additionally, Au, Hg and/or amalgam modified surfaces will be used for preparation of self-assembled monolayers with electrochemical detection. The nanostructures will be utilized for immobilization of detection probes, testing of chemical interactions after the surface modification and for improvement of sensitivity and selectivity of detection. Applications will be focused on biosensors of DNA damage and on research of proteins important in neurodegenerative diseases and cancer. (en)
- Cílem projektu je základní výzkum nanostrukturovaných povrchů pro elektronickou detekci biochemických interakcí. Využijeme zkušeností a know-how laboratoří obou partnerů k otevření nového směru výzkumu založého na nových metodách analýzy DNA a bílkovin s využitím nanotechnologií, mikrofluidiky a elektrochemie. Příprava a charakterizace nanostruktur bude směrována pro generování povrchem zesíleného analytického signálu s využitím nanoporézních substrátů, magnetických nanočástic, a uhlíkových nanotrubiček. Dále bude využito samouspořádaných monovrstev s povrchem modifikovaným Au, Hg, nebo amalgámy pro elektrochemickou detekci. Tyto nanostruktury budou využity pro imobilizaci detekčních sond, prozkoumání vlivu povrchové úpravy na chemické interakce a ke zlepšení citlivosti a selektivity detekce. Použití bude orientováno především na biosenzory pro detekci poškození DNA a výzkum bílkovin důležitých při progresi neurodegenerativních chorob a rakoviny.
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| Title
| - Nanotechnologies for protein and gene diagnostics (en)
- Nanotechnologie pro proteinovou a genovou diagnostiku
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| skos:notation
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| http://linked.open...avai/cep/aktivita
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| http://linked.open...kovaStatniPodpora
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| http://linked.open...ep/celkoveNaklady
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| http://linked.open...datumDodatniDoRIV
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| http://linked.open...i/cep/druhSouteze
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| http://linked.open...ep/duvernostUdaju
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| http://linked.open.../cep/fazeProjektu
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| http://linked.open...ai/cep/hlavniObor
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| http://linked.open...hodnoceniProjektu
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| http://linked.open...vai/cep/kategorie
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| http://linked.open.../cep/klicovaSlova
| - Diagnostics; DNA; electrochemistry; LIF; magnetic nanoparticles; microfluidics; proteins; self assembled monolayers (en)
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| http://linked.open...ep/partnetrHlavni
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| http://linked.open...inujicichPrijemcu
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| http://linked.open...cep/pocetPrijemcu
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| http://linked.open...ocetSpoluPrijemcu
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| http://linked.open.../pocetVysledkuRIV
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| http://linked.open...enychVysledkuVRIV
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| http://linked.open...lneniVMinulemRoce
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| http://linked.open.../prideleniPodpory
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| http://linked.open...iciPoslednihoRoku
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| http://linked.open...atUdajeProjZameru
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| http://linked.open.../vavai/cep/soutez
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| http://linked.open...usZobrazovaneFaze
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| http://linked.open...ai/cep/typPojektu
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| http://linked.open...ep/ukonceniReseni
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| http://linked.open.../cep/vedlejsiObor
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| http://linked.open...ep/zahajeniReseni
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| http://linked.open...jektu+dodavatelem
| - QD-protein conjugates and enzymatically modified nanolayers were prepared. Hg and solid amalgam electrodes with thiol nanolayers were invented and utilized in structure-sensitive analysis of proteins at picomole level. (en)
- Připraveny konjugáty QD-protein a nanovrstvy s kovalentně navázaným enzymem. Navrženy Hg a pevné amalgámové elektrody s thiolovými nanovrstvami a využity ke strukturně citlivé analýze proteinů v pikomolových množstvích. (cs)
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| http://linked.open...tniCyklusProjektu
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| http://linked.open.../cep/klicoveSlovo
| - Diagnostics
- DNA
- LIF
- electrochemistry
- magnetic nanoparticles
- microfluidics
- proteins
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| is http://linked.open...vavai/riv/projekt
of | - Electrochemical Renewal of Stationary Mercury Drop or Meniscus Electrodes
- Fifty years of nucleic acid electrochemistry
- Label-free electrochemical monitoring of DNA ligase activity
- Electrochemical stripping techniques in analysis of nucleic acids and their constituents
- End-labeling of peptide nucleic acid with osmium complex. Voltammetry at carbon and mercury electrodes
- Detection of abasic sites in DNA by electrochemical, immunoelectrochemical and acoustic methods using OsO4, 2,2,-bipyridine as a probe for unpaired thymine residues
- Electrochemical determination of thioredoxin redox states
- Fifty years of nucleic acid electrochemistry
- Proteins, electrochemical detection of
- Ionic strength-dependent structural transition of proteins at electrode surfaces
- Preparation of highly luminescent CdTe quantum dots and its probes
- CECE 2007. 4th international interdisciplinary meeting on bioanalysis
- CECE 2007. 4th international interdisciplinary meeting on bioanalysis
- Osmium Tetroxide, 2,2´-Bipyridine: Electroactive Marker for Probing Accessibility of Tryptophan Residues in Proteins
- Voltammetry of Osmium End-Labeled Oligodeoxynucleotides at Carbon, Mercury, and Gold Electrodes
- Electrocatalytic monitoring of metal binding and mutation-induced conformational changes in p53 at picomole level
- Electrocatalytic detection of polysaccharides at picomolar concentrations
- Bioaffinity magnetic reactor for peptide digestion followed by analysis using bottom-up shotgun proteomics strategy
- Kovalentní značení nukleosidů komplexy VIII- a VI- mocného osmia
- Electroactivity of nonconjugated proteins and peptides. Towards electroanalysis of all proteins
- Magnetic beads as versatile tools for electrochemical DNA and protein biosensing
- Capillary electrophoresis immunoassays with conjugated quantum dots
- Immunofluorescent labeling of tissues and free cells via QD-protein conjugates
- UV-LED photopolymerised monoliths
- Multidimensional liquid phase separations for mass spectrometry
- On-chip tryptic digest with indirect coupling to ESI-MS using magnetic particles
- Potential of quantum dots for single molecule detection in cell membranes and organelles
- CdTe quantum dots - preparation, physico-chemical properties and application as fluorescent markers
- Využití nanočástic ve fluorescenční mikroskopii a ramanově spektrometrii
- Preparation, physical-chemical properties and utilization of nanoparticles in bioanalysis
- Evanescent wave-initiated photopolymerisation as a new way to create monolithic open-tubular capillary columns: use as enzymatic microreactor for on-line protein digestion
- Electrophoresis today and tomorrow: helping biologists’ dreams come true
- Analyte transport in liquid junction nano-electrospray interface between capillary electrophoresis and mass spectrometry
- Fabrication and characterization of solid mercury amalgam electrodes for protein analysis
- Development of microfluidic analytical system for cell electrolysis
- Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries
- Autofocusing and ESI/MS analysis of protein digests in a miniaturized multicompartment electrolyzer
- Microdevices in mass spectrometry
- Zařízení pro připojení uzavřeného elektroforetického systému k hmotnostnímu spektrometru
- Zařízení pro připojení uzavřeného elektroforetického systému k hmotnostnímu spektrometru
- CECE 2008. 5th international interdisciplinary meeting on bioanalysis
- CECE 2008. 5th international interdisciplinary meeting on bioanalysis
- Miniaturization. Editorial. Electrophoresis 29 [24], p. 4807
- Changes in interfacial properties of alpha-synuclein preceding its aggregation
- CECE06. 3rd multidisciplinary meeting covering all aspects of modern bioanalysis
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| is http://linked.open...vavai/cep/projekt
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