About: Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation.

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About: Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Electroporation represents a powerful technique for cell transfection; however, its efficiency in haemopoietic cells (~1%) is largely unsatisfactory. Biological processes in haemopoietic cells are often studied using leukemia cell line HL-60. For this reason we developed conditions for efficiently introducing plasmids to HL-60 cells by electroporation, as an alternative to other techniques. This technique employs electric pulse (250-270 V; 1000 microF) followed by separation of living cells on Ficoll-Paque discontinuous gradient. Using 10-20 microg of plasmid, we routinely achieve 12-14% of transfectants. Electroporation represents a powerful technique for cell transfection; however, its efficiency in haemopoietic cells (~1%) is largely unsatisfactory. Biological processes in haemopoietic cells are often studied using leukemia cell line HL-60. For this reason we developed conditions for efficiently introducing plasmids to HL-60 cells by electroporation, as an alternative to other techniques. This technique employs electric pulse (250-270 V; 1000 microF) followed by separation of living cells on Ficoll-Paque discontinuous gradient. Using 10-20 microg of plasmid, we routinely achieve 12-14% of transfectants. (en)
Title	Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation. Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation. (en)
skos:prefLabel	Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation. Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation. (en)
skos:notation	RIV/68081707:_____/02:17023186!RIV/2003/GA0/A17003/N
http://linked.open.../vavai/riv/strany	154;156
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA524/99/0694), P(IBS5004009), P(KSK5011112), Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Janík, Robert Kozubík, Alois Bryja, Vítězslav Hofmanová, Jiřina Pacherník, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	641562
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/02:17023186
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	leukaemia cell line HL-60; electroporation; gene transfection (en)
http://linked.open.../riv/klicoveSlovo	electroporation gene transfection leukaemia cell line HL-60
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[E6F9EDD4D9AC]
http://linked.open...i/riv/nazevZdroje	Folia Biologica
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular and cellular mechanisms of important diseases Polyunsaturated fatty acids and cytokines - their role in maintenance of homeostasis at the cell population level Untraditional therapeutic approaches in oncology
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Bryja, Vítězslav Hofmanová, Jiřina Kozubík, Alois Pacherník, Jiří Janík, Robert
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors
issn	0015-5500
number of pages	3 (xsd:int)

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